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目的探讨(研究)锌-α 2-糖蛋白(ZAG)在成年雄性SD大鼠生殖系统中的表达情况。方法应用商品化试剂盒分别抽提RNA和蛋白质,利用实时荧光定量PCR(Realtime PCR)和免疫印迹(Westernblot)的方法研究ZAG在SD大鼠生殖系统各器官中的表达情况。结果雄性SD大鼠生殖系统各器官中均有ZAG蛋白的存在,不同组织表达有一定的差异,尤其是在尾部附睾及附睾脂肪组织中表达较高,其它组织相对较低。结论大鼠生殖系统中ZAG主要分布在尾部附睾及附睾脂肪组织。通过对ZAG蛋白在雄性SD大鼠生殖系统表达差异的研究,有助于进一步了解该蛋白在雄性生殖系统中的生物学功能。
Objective To investigate (research) the expression of zinc-α 2-glycoprotein (ZAG) in the reproductive system of adult male SD rats. Methods The commercial kit was used to extract RNA and protein respectively. Real-time PCR and Western blotting were used to study the expression of ZAG in various organs of the reproductive system of SD rats. Results There were some ZAG proteins in different parts of the reproductive system of male SD rats. The expression of ZAG protein was different in different tissues, especially in epididymal fat and epididymis. The other tissues were relatively low. Conclusion ZAG in the rat reproductive system is mainly distributed in the epididymal tail and epididymal adipose tissue. By studying the differences of ZAG expression in reproductive system of male SD rats, it is helpful to understand the biological function of ZAG protein in the male reproductive system.