目的　了解丙型肝炎病毒感染的不同人群血清 HCV RNA水平 ,比较定性和定量 PCR结果 ,探讨 HCV含量与血清 AL T的相关性 .方法　采用荧光定量 PCR和逆转录巢式定性 PCR同时检测 136例丙型肝炎病毒感染者血清 HCV RNA,并测定定量 PCR (+ )者血清 AL T.用相关系数分析 HCV RNA含量 Objective To understand the serum levels of HCV RNA in different populations infected with hepatitis C virus (HCV) and compare the results of qualitative and quantitative PCR to explore the correlation between HCV and serum ALT.Methods Fluorescence quantitative PCR and reverse transcriptase nested PCR were used to detect 136 HCV Hepatitis C virus infected patients with serum HCV RNA, and quantitative PCR (+) were determined serum AL T. Correlation coefficient analysis of HCV RNA content