产气荚膜杆菌能产生多种毒性物质,其中以α毒素较为多见,且其致病力亦较显著。在治疗与预防气性坏疽病时,都考虑到α毒素问题。因此,α毒素、抗毒素效价的测定,特别在研究气性坏疽免疫制品时,是必须解决的问题;这种免疫制品,在平战时均有其重要的意义。α毒素有致死、溶血和引起坏死的作用,利用这些特性,可作为α毒素效价测定的指标。过去用的方法主要有三种:小白鼠静脉注射测定法、试管中溶血活力测定法和豚鼠皮内坏死作用测定法。但用这些方法来测定α毒素还有许多缺点;用小白鼠静脉注射法测定毒性,除了许多不易避免的错误 Clostridium perfringens can produce a variety of toxic substances, including alpha toxin more common, and its virulence is also more significant. Alpha toxins are all considered in the treatment and prevention of gas gangrene. Therefore, the determination of alpha toxin and antitoxin potency, especially in the study of gas gangrene immunization products, is an issue that must be solved; such immune products have their important significance in peacetime. Alpha toxins are lethal, hemolysis and cause necrosis, the use of these characteristics can be used as an indicator of alpha toxin titer. There are three main methods used in the past: intravenous injection assay in mice, determination of hemolytic activity in test tubes and determination of intradermal necrosis in guinea pigs. However, there are a number of disadvantages associated with the determination of alpha toxins by these methods; the toxicity of the mice is determined by intravenous injection, with the exception of many unavoidable mistakes