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目的:采用立体定向技术建立Wistar大鼠额叶C6胶质瘤模型,观察成瘤动物的生物学特性,总结建立此模型的成功经验。方法:利用立体定向技术于纯种Wistar大鼠额叶接种C6胶质瘤细胞,观察大鼠的生物学特性,取脑标本行常规HE染色及胶质纤维酸性蛋白(GFAP)免疫荧光染色,观察肿瘤标本的病理特征和GFAP阳性情况。结果:大鼠脑内接种C6胶质瘤细胞的成功率为100%,成瘤大鼠的平均生存期为4周,肿瘤内部出现大片的坏死灶,坏死灶与肿瘤灶相间分布,采用免疫荧光检查发现,坏死灶不显影,而肿瘤灶呈条索状的免疫荧光条带。结论:立体定向技术可有效地建立Wistar大鼠额叶C6胶质瘤模型,其病理学特征符合胶质瘤的形态学特性,该模型在研究胶质瘤治疗方面有广泛的用途。
OBJECTIVE: To establish a model of frontal lobe C6 glioma in Wistar rats with stereotactic technique. The biological characteristics of the tumor-bearing animals were observed. The successful experience of establishing this model was summarized. Methods: C6 glioma cells were inoculated on the frontal lobe of Wistar rats using stereotactic technique. The biological characteristics of the rats were observed. HE staining and glial fibrillary acidic protein (GFAP) immunofluorescence staining were used to observe the brain samples. Pathological features of tumor specimens and GFAP positive cases. Results: The success rate of inoculation of C6 glioma cells in rat brain was 100%. The average survival time of tumor-bearing rats was 4 weeks. Large necrotic lesions were found in the tumor and the necrotic lesions were distributed with tumor focus. Immunofluorescence Examination found that necrotic lesions are not developed, while the tumor was cord-like immunofluorescence bands. Conclusion: The stereotactic technique can effectively establish the frontal cortex C6 glioma model in Wistar rats. Its pathological characteristics are in accordance with the morphological characteristics of glioma. This model has been widely used in the study of the treatment of glioma.