从SARS患者生前咽拭子和尸检肺组织分离病毒阳性的培养细胞超薄切片中,在电子显微镜下不仅发现了呼肠病毒,而且还查见具有超微结构形态特征的凋亡细胞。凋亡早期的感染细胞,其细胞核染色质浓缩、边集显著,胞浆内尚可查见病毒包涵体的残迹。多数凋亡细胞胞浆内未查见呼肠病毒及其包涵体。在早期,凋亡细胞胞核形状变化不大,核染色质呈不规则的边集、浓缩,或呈平台状。继而,凋亡细胞胞核形状高度不规则,染色质浓缩、致密,可见核孔残迹,但细胞轮廓尚保存完好,外周可查见含核质凋亡小体。到后期,凋亡细胞高度皱缩,核固缩并碎裂成细小的片块或颗粒,胞质稀少,胞体呈芽球状,细胞膜外围可查见大小不等、形状多样的含核质凋亡小体。此研究结果提示:与SARS相关呼肠病毒所诱导的细胞凋亡有可能在SARS的发病机制中起重要作用。 In the ultrathin sections of virus-positive cultured cells isolated from throat swabs and autopsy lungs of SARS patients, we not only found the reovirus under the electron microscope, but also found apoptotic cells with ultrastructural features. In the early stage of apoptosis, the infected cells were enriched in chromatin of the nucleus, the marginal set was significant, and the remains of the virus inclusion body were still found in the cytoplasm. Most apoptotic cells did not find cytoplasm reovirus and its inclusion bodies. In the early stage, the shape of apoptotic cells did not change much, nuclear chromatin showed irregular edge set, concentrated, or in the form of platform. Subsequently, the apoptotic cells were highly irregular in nuclear shape. The chromatin was dense and dense, showing the appearance of nuclear pores. However, the outline of the cells remained intact, and apoptotic bodies with nuclei could be found in the periphery. In the late stage, apoptotic cells were highly shrunken, pyknotic and fragmented into fine pieces or granules with sparse cytoplasm and sprouted globular cells. The periphery of the cell membrane could be seen in varying sizes, with various shapes containing apoptotic nuclei Small body. The results suggest that the apoptosis induced by reovirus associated with SARS may play an important role in the pathogenesis of SARS.