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目的探讨荧光原位杂交检测乳腺浸润性导管癌人类表皮生长因子受体-2(HER-2)基因表达及其与特异AT序列结合蛋白1(SATB1)、激素受体(HR)蛋白表达关系。方法选取乳腺浸润性导管癌手术患者72例,术中采集乳腺癌组织标本,免疫组化法(IHC)检测HER-2、SATB1和HR蛋白表达,采用荧光原位杂交法(FISH)检测HER-2基因扩增。结果 FISH检测HER-2基因扩增率为38.89%(28/72),IHC检测HER-2蛋白阳性率为22.22%(16/72),两组比较差异有统计学意义(P<0.05);HER-2基因扩增(+)组SATB1阳性率明显高于HER-2基因扩增(-)组(P<0.05),HR阳性率明显低于HER-2基因扩增(-)组(P<0.05)。经Pearson相关性分析,HER-2基因扩增与HR蛋白表达呈负相关(r=-0.405,P<0.05),与SATB1蛋白表达呈正相关(r=0.396,P<0.05);HER-2基因扩增与组织学分级、淋巴结转移和TNM分期紧密相关,其中组织学Ⅲ级和临床Ⅲ期者HER-2基因扩增阳性率明显高于组织学Ⅰ、Ⅱ级和临床Ⅰ、Ⅱ期,有淋巴结转移者HER-2基因扩增阳性率明显高于无淋巴结转移者(P<0.05)。HER-2基因扩增与年龄、肿瘤直径大小无明显相关性(P>0.05)。结论荧光原位杂交检测HER-2基因扩增对乳腺浸润性导管癌诊断率高,且与SATB1、HR蛋白表达紧密相关,三者可预示临床分期和淋巴结转移特征。
Objective To investigate the expression of human epidermal growth factor receptor-2 (HER-2) gene and its relationship with specific AT sequence binding protein 1 (SATB1) and hormone receptor (HR) protein in invasive ductal carcinoma of the breast by fluorescence in situ hybridization. Methods Totally 72 patients with breast invasive ductal carcinoma were enrolled in this study. Breast cancer tissues were collected during the operation. The expressions of HER-2, SATB1 and HR proteins were detected by immunohistochemical staining (IHC) 2 gene amplification. Results The positive rate of HER2 gene detected by FISH was 38.89% (28/72), and the positive rate of HER2 detected by IHC was 22.22% (16/72). The difference between the two groups was statistically significant (P <0.05). The positive rate of SATB1 in HER-2 gene amplification group was significantly higher than that in HER-2 gene amplification group (P <0.05), and the positive rate of HR in HER-2 gene amplification group was significantly lower than that in HER-2 gene amplification group <0.05). The Pearson correlation analysis showed that HER-2 gene amplification was negatively correlated with HR protein expression (r = -0.405, P <0.05) and positively correlated with SATB1 protein expression (r = 0.396, P < The amplification was closely related to histological grade, lymph node metastasis and TNM staging. The positive rate of HER-2 gene amplification in histological grade Ⅲ and clinical stage Ⅲ was significantly higher than that in histological grade Ⅰ, Ⅱ and clinical Ⅰ, Ⅱ The positive rate of HER-2 gene amplification in lymph node metastasis was significantly higher than those without lymph node metastasis (P <0.05). There was no significant correlation between HER-2 gene amplification and age and tumor diameter (P> 0.05). Conclusion The detection of HER-2 gene by fluorescence in situ hybridization has a high diagnostic value for breast invasive ductal carcinoma, and is closely related to the expression of SATB1 and HR protein. The three may predict clinical stage and lymph node metastasis.