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AIM:To study the effect of matrine on activation of Kupffercell during cold ischemia and reperfusion injury in ratorthotopic liver transplantation(OLT).METHODS:168 syngeneic SD rats were randomly dividedinto four groups:untreated group,small-dose treated group,large-dose treated group and sham operation group.After5 hours of preservation in Ringer’s(LR)solution,orthotopicimplantation of the donor liver was performed.At 1 h,2 h,4 h and 24 h after reperfusion of the portal vein,6 rats werekilled in each group to collect the serum and the liver forassay and pathology.RESULTS:Matrine markedly inhibited the activation of Kupffercells and their release of tumor necrosis factor(TNF).TNFcytotoxicity level at 2 h decreased significantly by matrinetreatment(7.94±0.42,2.39±0.19 and 2.01±0.13 U/ml,respectively;P<0.01),so did the other three time points.Thelevel of hylluronic acid(HA)and alanine transaminase(ALT)decreased significantly in both treated groups,and matrinetreatment markedly ameliorated focal necrosis of hepatocytes,inflammatory cells aggregating,rounding and detachment ofsinusoidal endothelial cells(SEC).And no significant differencewas observed between the treated groups.CONCLUSION:Matrine can inhibit the activation of Kupffercell and prevent the donor liver from cold preservation andreperfusion injury in rat orthotopic liver transplantation.
AIM: To study the effect of matrine on activation of Kupffercell during cold ischemia and reperfusion injury in ratorthotopic liver transplantation (OLT). METHODS: 168 syngeneic SD rats were randomly divided into four groups: untreated group, small-dose treated group, large-dose treated group and sham operation group. After 5 hours of preservation in Ringer’s (LR) solution, orthotopicimplantation of the donor liver was performed. At 1 h, 2 h, 4 h and 24 h after reperfusion of the portal vein, 6 rats werekilled in each group to collect the serum and the liver forassay and pathology .RESULTS: Matrine markedly inhibited the activation of Kupffer cells and their release of tumor necrosis factor (TNF). TNF.totoxicity level at 2 h markedly reduced by matrinetreatment (7.94 ± 0.42, 2.39 ± 0.19 and 2.01 ± 0.13 U / ml, respectively; P <0.01), so did the other three time points. The level of hylluronic acid (HA) and alanine transaminase (ALT) decreased significantly in both treated groups, and matrinetreatment markedly amelior ated focal necrosis of hepatocytes, inflammatory cells aggregating, rounding and detachment of sinusoidal endothelial cells (SEC). No significant difference was observed between the treated groups. CONCLUSION: Matrine can inhibit the activation of Kupffer cells and prevent the donor liver from cold preservation and reperfusion injury in rat orthotopic liver transplantation.