目的 :采用分子杂交及PCR方法 ,分析鸭α 干扰素基因的表达及多态性。方法 :从鸭外周血分离出的单核细胞在体外经PHA(5 μg/ml)刺激不同时间后 ,提取总RNA。以RT PCR方法检测鸭α 干扰素 (DuIFN α)mRNA表达状况。引物根据最近公布的DuIFN α基因序列设计。从鸭外周血单核细胞中提取的基因组DNA经限制性内切酶BamHI,HindIII,PstI,XbaI消化后 ,以公布的DuIFN α序列为探针 ,采用Southern杂交分析DuIFN α基因的多样性。结果 :在未经PHA刺激的鸭外周血单核细胞 (PBMCs)中 ,未检测到DuIFN α表达 ;PHA刺激 4h后 ,即可检测到DuIFN α表达 ,一直持续到 2 4h。基因组DNA限制性内切酶多态性分析表明 ,PstI酶切后 ,出现片段大小各异的杂交信号 ,提示DuIFN α存在多样性。结论 :鸟类α 干扰素基因与哺乳动物类似 ,也具有多样性 Objective: To analyze the expression and polymorphism of duck interferon-alpha gene by molecular hybridization and PCR. Methods: Monocytes isolated from peripheral blood of duck were extracted from total RNA after PHA (5 μg / ml) in vitro stimulated for different time. The expression of duck interferon-alpha (DuIFNα) mRNA was detected by RT-PCR. Primers are designed based on the recently published DuIFN a gene sequence. The genomic DNA extracted from duck peripheral blood mononuclear cells was digested with restriction enzymes BamHI, HindIII, PstI and XbaI. The published DuIFNα sequence was used as a probe to analyze the diversity of DuIFNα gene by Southern blot. RESULTS: No expression of DuIFNα was detected in duck peripheral blood mononuclear cells (PBMCs) without PHA stimulation. DuIFNα expression was detected after 4 h of PHA stimulation and continued until 24 h. Genomic DNA restriction endonuclease polymorphism analysis showed that PstI digestion, there are fragments of different sizes hybridization signal, suggesting that DuIFN α diversity. Conclusion: The avian interferon alpha gene is similar to mammals and has a diversity