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目的研究细粒棘球蚴慢性感染阶段小鼠血清中细胞因子水平的变化,探索机体对抗寄生虫感染的免疫应答机制。方法从病羊内脏分离细粒棘球绦虫原头节,注射入BALB/c小鼠腹腔(2 000个原头节/只),建立细粒棘球蚴感染小鼠模型,对照组小鼠腹腔注射等体积PBS。于感染5个月后,采集对照及感染组小鼠血清,采用流式液相多重蛋白定量技术检测血清中多种细胞因子水平并分析。结果感染组小鼠腹腔、肝脏、肺脏等部位均出现多个单一性包囊;其血清中IL-17A、IL-6、IFN-γ、MCP-1、IL-12P70、TNF-α等炎症因子水平均显著高于对照组(t=2.713~9.255,P均<0.05);而抑炎因子IL-10水平亦显著升高(t=3.936,P<0.001)。结论细粒棘球蚴慢性感染阶段小鼠体内炎症因子水平较高,有助于抑制虫体生长。
Objective To study the changes of cytokines in serum of mice with chronic infection of Echinococcus granulosus and to explore the immune response mechanism of the body against parasitic infections. Methods The murine model of Echinococcus granulosus infection was established by injecting the progenitor cells of Echinococcus granulosus into the peritoneal cavity of the BALB / c mice (2 000 prions / day) An equal volume of PBS is injected. After 5 months of infection, the sera of control and infected mice were collected, and the levels of various cytokines in serum were detected by flow cytometry and multiple protein quantitative analysis. Results A number of single cysts were found in the peritoneal, liver and lung of infected mice. The inflammatory factors such as IL-17A, IL-6, IFN-γ, MCP-1, IL-12P70 and TNF- (T = 2.713 ~ 9.255, P <0.05), while the level of IL-10 was also significantly increased (t = 3.936, P <0.001). Conclusion Echinococcus granulosus chronic infection stage mice with high levels of inflammatory cytokines, help to inhibit the growth of parasites.