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目的：探索大鼠嗅鞘细胞（olfactory ensheathing cells，OECs）移植联合督脉电针对大鼠脊髓损伤（spinal cord injury SCI）后水通道蛋白-4（AQP-4）和后肢功能的影响。方法：取Wistar大鼠150只，随机分为正常组(50只)、OECs移植组(50只)、OECs移植联合督脉电针组(50只)，OECs移植联合督脉电针组和OECs移植组用改良的Allen法制成脊髓损伤模型，造模成功后， OECs移植组和OECs移植联合督脉电针在损伤处移植嗅鞘细胞。于术后1、3、7、14、21、28天进行BBB (Basso-Beattle-Bresnahan)运动功能评分，应用免疫组织化学技术检测脊髓组织AQP-4的表达，并用图像分析仪进行定量分析。结果：术后3～28天，OECs移植联合督脉电针组的BBB评分较OECs移植组明显提高,术后第1天，联合组和OECs移植组受损脊髓灰质、白质中AQP-4的表达明显增加；第3天时均达到高峰，但联合组低于O E C s移植组(P<0.05)。第7、14、21、28天，与O E C s移植组比较，联合组A Q P-4表达也较低(P<0.01)。结论：O E C s移植联合督脉电针使脊髓损伤后A Q P-4表达减少，这可能更有利于抑制脊髓水肿、消除脊髓继发性损伤，保存了残存正常脊髓组织并促进神经轴突再生，改善其肢体运动功能。“,”Objective To investigate effects of combination of transplanted olfactory ensheathing cells(OECs) and Goremor vessel electroacupuncture on the water channel aquaporin-4(AQP-4) expression and hind limbs function recovery in experimental spinal cord injured rats.Methods One hundred and fifty Wistar rats were divided into the normal group, the OECs grafted group(OECs group) and the OECs grafted plus Goremor vessel electroacupuncture group(OECs+EC group), with 50 rats in each group, modified Allen method was used to establish spinal cord injury model in the OECs and OECs+EC group. OECs were grafted into the transected site of spinal cord in OECs group and OECs+EC group after modelling successfully. At the 1st, 3rd, 7th, 14th 21th and 28th day after injury, the hind limbs function of rats were estimated with system of the BBB locomotion score respectively, and the AQP-4 expression in spinal cord tissue was determined by immunohistochemistry technique and quantity analysed with image analyzer.Results From 3 days to 28 days, the BBB locomotion scores of OECs+EC groups was higher than that in the OECs group(P<0.05),At the 1st day after spinal cord injury, the AQP-4 expression was significantly increased in gray matter and white matter of spinal cord in the OECs group and the OECs+EC group, which reached the peak at the 3rd day, but showed significant difference between the two groups (P<0.05), and the difference at the 7th, 14th 21th and 28th day became more significant (P<0.01).Conclusion Combination of transplanted olfactory ensheathing cells(OECs) and Goremor vessel electroacupuncture can down-regulate AQP-4 expression after spinal cord injury, inhibits pinal cord edema for alleviating secondary spinal cord lesion, so as to protect the residual normal spinal cord tissues and promote the regeneration of axons, and enhance the recovery of locomotion function.