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目的:建立在同一色谱条件下同时测定丹参多组分复合微乳中丹参酮ⅡA和丹酚酸B的HPLC含量测定方法。方法:采用Kromasil C18(250mm×4.6mm,5μm)色谱柱,以乙腈-0.4%磷酸溶液(0-20min,10-45∶90-55;20-21min,45-80∶55-20;21-37min,80∶20;37-38min,80-10∶20-90,38-43min,10∶90)为流动相,流速1.2m L/min,丹参酮ⅡA和丹酚酸B的检测波长分别为270、286nm,进样量10μL。结果:丹参酮ⅡA和丹酚酸B分别在0.3-64μg/m L和10-210μg/m L范围内线性关系良好,加样回收率RSD均小于3%,以标示量计算的平均含量分别为(957.17±1.04)μg/g和(1 315.08±15.66)μg/g。结论:本方法简便、稳定、用时较少。该色谱条件下,各成分分离度良好,可用于丹参复合微乳的含量测定。
Objective: To establish a method for the simultaneous determination of tanshinone ⅡA and salvianolic acid B in Salvia miltiorrhiza multi-component microemulsion under the same chromatographic conditions. Methods: A Kromasil C18 (250 mm × 4.6 mm, 5 μm) column was used with acetonitrile-0.4% phosphoric acid (0-20 min, 10-45: 90-55; 20-21 min, 45-80: 37 min, 80:20; 37-38 min, 80-10:20-90, 38-43 min, 10:90) as the mobile phase at a flow rate of 1.2 m L / min. The detection wavelengths of tanshinone IIA and salvianolic acid B were 270 , 286nm, injection volume 10μL. Results: Tanshinone Ⅱ A and salvianolic acid B showed good linearity in the range of 0.3-64 μg / mL and 10-210 μg / mL, respectively. The RSDs of recoveries were all less than 3%. The average contents of tanshinone ⅡA and salvianolic acid B were ( 957.17 ± 1.04) μg / g and (1 315.08 ± 15.66) μg / g. Conclusion: This method is simple, stable, with less time. The chromatographic conditions, the various components of good separation, can be used for the determination of Danshen composite microemulsion.