论文部分内容阅读
目的:探索未成熟卵子的最佳冷冻时期。方法:收集卵胞质内单精子显微注射-胚胎移植(ICSI-ET)周期中未成熟的卵母细胞,按其成熟度分为生发泡期(GV组)卵子179枚和第一次减数分裂中期(MI组)卵子323枚,所有卵子均经玻璃化冷冻,解冻后行体外成熟(IVM)培养,ICSI受精,观察比较GV组和MI组解冻后存活、体外成熟、受精及胚胎发育情况。结果:GV组复苏存活率显著高于MI组(83.24%vs 75.54%,P=0.045),MI组体外成熟率、受精率、卵裂率、优质胚胎率均略均高于GV组,但无统计学差异(P>0.05),MI组可利用胚胎率显著高于GV组(78.67%vs 60.53%,P=0.041)。结论:超促排卵周期中未成熟卵母细胞先玻璃化冷冻保存,再行体外培养是可行的。GV期卵母细胞复苏存活率高于MI期卵母细胞,但MI期卵母细胞冻融后发育潜能优于GV组卵母细胞。
Objective: To explore the optimal freezing period of immature eggs. Methods: The immature oocytes in the intracytoplasmic sperm injection-embryo transfer (ICSI-ET) cycle were collected and divided into 179 mature oocytes (GV group) and the first subtraction All the oocytes were vitrified and frozen in vitro. All the oocytes were cultured in vitro (IVM) after thawing, ICSI fertilized. The survival, in vitro maturation, fertilization and embryonic development of the GV and MI groups were observed after thawing . Results: The survival rate of resuscitation in GV group was significantly higher than that in MI group (83.24% vs 75.54%, P = 0.045). The in vitro maturation rate, fertilization rate, cleavage rate and quality embryo rate in MI group were slightly higher than those in GV group (P> 0.05). The available embryo rate in MI group was significantly higher than that in GV group (78.67% vs 60.53%, P = 0.041). Conclusion: Immature oocytes in the ovarian hyperstimulation cycle are vitrified and frozen before being cultured in vitro. The survival rate of GV oocytes recovery was higher than that of MI oocytes, but the developmental potential of MI oocytes after freezing and thawing was better than that of GV oocytes.