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[目的]研究胆胃胶囊对胆汁反流性胃炎模型的作用机制。[方法]SD大鼠72只随机分为6组,达喜阳性药组,胆胃胶囊大、中、小剂量组,另设假手术对照空白组和模型组比较,按组分别空腹灌胃给药,每日1次,连续给药14 d。第14天给药后取胃称重,以胃脏器系数代表水肿程度,并取贲门至幽门胃黏膜皮部和腺部做病理检查。免疫组化检测癌基因蛋白表达。[结果]模型组与空白组比较,胃脏器系数明显增加,表明造模成功。与模型组比较,阳性药组、胆胃胶囊大、中剂量组胃脏器系数明显减小。电镜观察,阳性药组与胆胃胶囊大、中剂量组显著减轻基底细胞增厚及腺上皮充血水肿;阳性药组与胆胃胶囊大剂量组也能明显改善黏液细胞下降及核大深染现象。差异均有统计学意义(P<0.05-<0.01)。胆胃胶囊大、中剂量组表皮生长因子受体表达明显增强,p21表达明显下降。[结论]胆胃胶囊能显著抑制胃黏膜充血、水肿,减轻基底细胞增厚和黏液细胞下降,上调胃黏膜表皮生长因子受体表达及下调p21表达。
[Objective] To study the mechanism of gall bladder capsule on the mechanism of bile reflux gastritis. [Methods] Seventy-two SD rats were randomly divided into 6 groups, Daxi positive drug group, Daweiwei capsule large, middle and small dose groups. Another sham-operated control blank group and model group were compared, and each group was given fasting by oral gavage. The drug was administered once daily for 14 days. On the 14th day after administration, the stomach was weighed and the gastric organ coefficient was used to represent the degree of edema. From the cardia to the pyloric gastric mucosa, the skin and glands were examined for pathology. Immunohistochemical detection of oncogene protein expression. [Results] Compared with the blank group, the gastric organ coefficient was significantly increased in the model group, indicating that the model was successful. Compared with the model group, the gastric organ coefficient was significantly decreased in the positive drug group and the large and middle dose group of the Shiwei capsule. Electron microscopy showed that the positive drug group and the large and middle dose group of the Weiwei capsule significantly reduced the basal cell thickening and hyperemia and edema of the glandular epithelium; the positive drug group and the large dose of the Ganwei capsule also significantly improved the phenomenon of the mucinous cell decline and deep nuclear staining. . The differences were statistically significant (P<0.05-<0.01). The expression of epidermal growth factor receptor in the large and middle dose group of Biao capsule was significantly increased, and p21 expression was significantly decreased. [Conclusion] Bile gastric capsule can significantly inhibit gastric mucosal hyperemia and edema, reduce basal cell thickening and mucus cell decline, up-regulate epidermal growth factor receptor expression in gastric mucosa and down-regulate p21 expression.