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目的 观察肺炎支原体膜脂蛋白 (Mp LAMP)对THP 1 (前单核白血病细胞系 )细胞TLR2 (Toll样受体 )与mICAM 1 (膜结合型细胞间黏附分子 1 )表达的影响 ,探讨膜脂蛋白中的活性成分及二者表达的相互关系。方法 用流式细胞术检测Mp LAMP刺激组、抗人TLR2功能纯化抗体(TL2 .1 )阻断组及消化酶处理组的THP 1细胞mICAM 1和TLR2的表达水平。结果 与对照组相比 ,不同刺激浓度的Mp LAMP对THP 1细胞膜上TLR2及mICAM 1的表达均有明显的上调作用 (P <0 .0 5 ) ,且表达水平的增加对Mp LAMP的刺激具有浓度依耐性 ;用TL2 .1将THP 1细胞膜上的TLR2阻断后 ,Mp LAMP对mICAM 1表达的诱导作用明显受到抑制 (P <0 .0 1 ) ,其阻断效果随TL2 .1量的增加而加强 ;酶消化试验证实Mp LAMP的活性成分是其脂质部分。相关分析表明 ,mICAM 1在THP 1细胞上的表达水平与TLR2密切相关 (r=0 .989,P <0 .0 1 )。结论 肺炎支原体膜脂蛋白通过TLR2介导上调THP 1细胞mICAM 1的表达 ,在一定程度上影响着肺炎支原体感染所导致的炎性反应的强弱。
Objective To investigate the effect of Mycoplasma pneumoniae membrane lipoprotein (Mp LAMP) on the expression of TLR2 (Toll-like receptor) and mICAM 1 (membrane-associated intercellular adhesion molecule 1) in THP 1 (precytokine cell line) Interaction between active ingredients in proteins and their expression. Methods The expressions of mICAM 1 and TLR2 in THP 1 cells were detected by flow cytometry in Mp LAMP stimulation group, anti-human TLR2 functional purified antibody (TL2. 1) blocking group and digestive enzyme treatment group. Results Compared with the control group, Mp LAMP at different concentrations had a significant up-regulation of TLR2 and mICAM1 expression on THP1 cells (P <0.05), and the increase of Mp LAMP stimulation had Concentration dependent on patience; with TL2 .1 THP1 cell membrane TLR2 blocked, Mp LAMP on mICAM1 expression was significantly inhibited (P <0.01), the blocking effect with the amount of TL2 .1 Increase and strengthen; enzyme digestion test confirmed that the active component of Mp LAMP is its lipid part. Correlation analysis showed that the expression level of mICAM 1 in THP 1 cells was closely related to TLR2 (r = 0.989, P <0.01). Conclusion Mycoplasma pneumoniae membrane lipoprotein up-regulates the expression of mICAM-1 in THP-1 cells through TLR2, to a certain extent, affects the intensity of inflammatory response caused by Mycoplasma pneumoniae infection.