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目的:观察知柏地黄汤对解脲脲原体(UU)感染模型大鼠精子线粒体呼吸链复合物的影响。方法:90只雄性SD大鼠,随机分成空白对照组、模型对照组、中药组(知柏地黄汤8.56 g生药/(kg·d))、西药组[强力霉素20 mg/(kg·d)]、中西药组(知柏地黄汤+强力霉素等效剂量之和),除空白对照组外,其他组均采用经大鼠膀胱注射解脲脲原体建立UU感染动物模型,然后,连续灌胃21 d(各组给予对应药物)后处死,取附睾标本检测精子质量,分光光度法检测精子线粒体呼吸链复合物(Ⅰ、Ⅱ、Ⅲ、Ⅳ)活性。结果:①模型对照组、空白对照组、中药组、西药组、中西药组建模10 d后UU培养阳性率分别为92.9%、0%、33.3%、26.7%、20.0%,模型对照组UU阳性率明显高于其他治疗组(P<0.05)。②模型对照组、空白对照组、中药组、西药组、中西药组大鼠附睾精子浓度分别为(0.97±0.23)、(3.02±0.52)、(1.21±0.35)、(1.02±0.31)、(1.52±0.28)×10~6/ml,活动率分别为(58.62±15.36)%、(80.45±7.21)%、(75.52±8.78)%、(68.43±10.25)%、(78.25±7.67)%,a+b级精子百分率分别为(6.15±1.02)%、(10.32±1.14)%、(10.12±1.08)%、(9.01±1.27)%、(10.74±1.03)%;模型对照组大鼠附睾精子浓度、活动率及a+b级精子百分率明显低于空白对照组(P<0.01);各治疗组大鼠附睾精子活动率及a+b级精子百分率明显高于模型对照组(P<0.01)。③模型对照组、空白对照组、中药组、西药组、中西药组大鼠精子线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ活性分别为(31.54±16.25)、(136.86±6.34)、(100.68±14.41)、(81.68±6.78)、(124.06±5.54)μmol/(min·mg),(9.50±3.86)、(20.34±0.37)、(10.88±1.04)、(12.93±1.07)、(16.23±0.60)μmol/(min·mg),(5.58±1.79)、(19.60±0.61)、(11.34±1.35)、(13.87±1.23)、(15.96±0.69)μmol/(min·mg),(9.54±1.34)、(28.98±3.33)、(17.02±2.04)、(18.41±2.67)、(21.66±2.93)μmol/(min·mg)。模型对照组大鼠精子线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ活性明显低于空白对照组(P<0.01);中药组、西药组、中西药组大鼠精子线粒体呼吸链复合物Ⅰ、Ⅲ、Ⅳ活性明显高于模型对照组(P<0.01),西药组、中西药组大鼠精子线粒体呼吸链复合物Ⅱ活性高于模型对照组(P<0.05)。结论:知柏地黄汤能够提高UU感染大鼠精子质量及线粒体呼吸链复合物活性。这可能是知柏地黄汤治疗UU感染所致不育症的机制之一。
Objective: To observe the effect of Zhibei Dihuang decoction on sperm mitochondrial respiratory chain complex in UU-infected rats. Methods: Ninety male Sprague-Dawley rats were randomly divided into control group, model group, TCM group (8.56 g crude drug / (kg · d)), Western medicine group (doxycycline 20 mg / (kg · d) ), Western medicine group (Zhibo Di Huang Tang + doxycycline equivalent dose), except for the blank control group, the other groups were used to establish UU infection rat bladder model of UU infection in the bladder, and then, The rats were sacrificed on the 21th day after administration of the corresponding drugs. The epididymis was used to detect the sperm quality and the activity of sperm mitochondrial respiratory chain complex (Ⅰ, Ⅱ, Ⅲ, Ⅳ) was detected by spectrophotometry. Results ① The positive rates of UU culture in model control group, blank control group, traditional Chinese medicine group, western medicine group and western medicine group were 92.9%, 0%, 33.3%, 26.7% and 20.0% respectively after 10 days of modeling. The UU The positive rate was significantly higher than other treatment groups (P <0.05). ② The epididymal sperm concentrations of model control group, blank control group, traditional Chinese medicine group, western medicine group and western medicine group were (0.97 ± 0.23), (3.02 ± 0.52), (1.21 ± 0.35), (1.02 ± 0.31), 1.52 ± 0.28) × 10 ~ (6) / ml, and the activity rates were (58.62 ± 15.36)%, (80.45 ± 7.21)%, (75.52 ± 8.78)%, (68.43 ± 10.25)% and The percentages of a + b grade sperm were (6.15 ± 1.02)%, (10.32 ± 1.14)%, (10.12 ± 1.08)%, (9.01 ± 1.27)% and (10.74 ± 1.03)%, respectively (P <0.01). The sperm motility and the percentage of a + b sperm in epididymis of rats in each treatment group were significantly higher than those in model control group (P <0.01) . ③ The activity of mitochondrial respiratory chain complex Ⅰ, Ⅱ, Ⅲ and Ⅳ in model control group, blank control group, traditional Chinese medicine group, western medicine group and western medicine group were (31.54 ± 16.25), (136.86 ± 6.34), (100.68 ± 14.41, (81.68 ± 6.78), (124.06 ± 5.54) μmol / (min · mg), (9.50 ± 3.86), (20.34 ± 0.37), (10.88 ± 1.04), (12.93 ± 1.07), (16.23 ± 0.60), (5.58 ± 1.79), (19.60 ± 0.61), (11.34 ± 1.35), (13.87 ± 1.23), (15.96 ± 0.69) μmol / (min · mg) 1.34), (28.98 ± 3.33), (17.02 ± 2.04), (18.41 ± 2.67) and (21.66 ± 2.93) μmol / (min · mg), respectively. The activity of mitochondrial respiratory chain complex Ⅰ, Ⅱ, Ⅲ and Ⅳ in model control group was significantly lower than that of the blank control group (P <0.01); the sperm mitochondrial respiratory chain complex Ⅰ, (P <0.01). The activities of sperm mitochondrial respiratory chain complex Ⅱ in western medicine group and western medicine group were higher than those in model control group (P <0.05). Conclusion: Zhibai Dihuang Decoction can improve sperm quality and mitochondrial respiratory chain complex activity in UU-infected rats. This may be Zhibei Di Huang Tang UU infection caused by infertility caused by one of the mechanisms.