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建立了超高效液相色谱法测定灯盏细辛中的飞蓬苷(1)、绿原酸(2)、野黄芩苷(3)、3,5-O-二咖啡酰基奎宁酸(4)、灯盏甲素(5)和4,5-O-二咖啡酰基奎宁酸(6)的含量。采用BEH C18色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,多波长检测[260 nm(1),326 nm(2、4、6),335 nm(3、5)]。1～6分别在0.025～0.8、0.005～0.16、0.013～0.4、0.015～0.48、0.008～0.24和0.005～0.16 mg/ml范围内线性关系良好;平均回收率分别为97.2%、96.8%、100.1%、99.5%、99.2%和101.2%,RSD分别为2.1%、1.7%、1.9%、2.6%、2.5%和2.3%。
To establish a HPLC method for the determination of eupatoride (1), chlorogenic acid (2), scutellarin (3), 3,5-O-dicaffeoylquinic acid (4) The content of the scutellarin (5) and 4,5-O-disofoacylquinic acid (6). A BEH C18 column was used with gradient elution of acetonitrile-0.1% phosphoric acid as the mobile phase and multi-wavelength detection [260 nm (1), 326 nm (2, 4, 6), 335 nm (3, 5]]. 1 to 6 showed good linearity in the range of 0.025-0.8,0.005-0.16,0.013-0.4,0.015-0.48,0.008-0.24 and 0.005-0.16 mg / ml respectively; the average recoveries were 97.2%, 96.8% and 100.1%, respectively , 99.5%, 99.2% and 101.2% with RSD of 2.1%, 1.7%, 1.9%, 2.6%, 2.5% and 2.3%, respectively.