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目的考察Ⅱ型ZTC1+1天然澄清剂在黄精多糖提取中的纯化效果。方法以固形物去除率、多糖保留率为指标,通过正交试验进行方法优化。结果Ⅱ型ZTC1+1天然澄清剂能有效纯化多糖,并与澄清剂的加入量显著相关。结论Ⅱ型ZTC1+1天然澄清剂处理黄精多糖的最佳工艺为:在药液浓度为1:10的情况下,加入配制好的Ⅱ型ZTC1+1天然澄清剂B组分使其体积占溶液总体积的2%,搅拌10 min,60℃水浴保温50 min;再加入A组分使其占总体积的1%,搅拌10 min,60℃水浴保温50 min;静置12 h,过滤,即得。
Objective To investigate the purification effect of type Ⅱ ZTC1 + 1 natural clarifying agent on the extraction of Polygonatum. Methods The removal rate of solid, polysaccharide retention rate as an indicator, by orthogonal test method optimization. Results Type Ⅱ ZTC1 + 1 natural clarifier can effectively purify polysaccharides, and was significantly correlated with the addition of clarifier. CONCLUSION: The best technology for treating natural polysaccharides with type Ⅱ ZTC1 + 1 natural clarifier is as follows: when the solution concentration is 1:10, the prepared type Ⅱ ZTC1 + 1 natural clarifying agent B is added to make the volume of solution The total volume of 2%, stirring 10 min, 60 ° C water bath for 50 min; then add A component to account for 1% of the total volume, stirring 10 min, 60 ° C water bath for 50 min; Got it.