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目的：探讨血浆内皮素１（ＥＴ１）、血小板聚集及血小板内游离Ｃａ２＋在慢性肺原性心脏病（肺心病）急性加重期的意义及其相互关系。方法：用放射免疫分析法测定两组（４２例慢性肺心病患者急性加重期和２５例健康者）血浆ＥＴ１含量，用比浊法测定两组血小板聚集率和用Ｆｕｒａ２／ＡＭ荧光技术测定血小板内游离Ｃａ２＋含量。结果：慢性肺心病急性加重期ＥＴ１水平明显升高（Ｐ＜０．０１），血小板聚集率增高（Ｐ＜０．０１），血小板内游离Ｃａ２＋含量显著增加（Ｐ＜０．０１）。另外还观察到ＡＤＰ诱导的血小板聚集率与血小板内游离Ｃａ２＋含量之间呈正相关（ｒ＝０．７３８，Ｐ＜０．０１），而血浆ＥＴ１水平与血小板聚集率和血小板内游离Ｃａ２＋含量之间无明显相关性。结论：血浆ＥＴ１在慢性肺心病急性加重期的发病中可能起一定作用。此期血小板的功能处于活跃状态，易于聚集，形成血栓，加重病情；而血浆ＥＴ１对血小板功能可能无影响。
Objective: To investigate the significance and relationship of plasma endothelin 1 (ET1), platelet aggregation and platelet intracellular free Ca2 + in acute exacerbation of chronic pulmonary heart disease (pulmonary heart disease). Methods: The levels of plasma ET1 in two groups (42 exacerbations and 25 healthy subjects) were determined by radioimmunoassay. The levels of platelet aggregation were measured by turbidimetric method and the levels of platelet aggregation were measured by Fura2 / AM fluorescence assay Free Ca2 + content. Results: The levels of ET1 in acute exacerbation of chronic pulmonary heart disease were significantly increased (P <0.01), the platelet aggregation rate was increased (P <0.01), and the content of free Ca2 + in platelet significantly increased (P <0.01). In addition, there was a positive correlation between ADP-induced platelet aggregation and free intracellular Ca2 + (r = 0.738, P <0.01), while plasma levels of ET1, platelet aggregation and free intracellular Ca2 + No significant correlation. Conclusion: Plasma ET1 may play a role in the pathogenesis of acute exacerbation of chronic pulmonary heart disease. Platelet function during this period in an active state, easy to gather, the formation of thrombus, aggravate the condition; and plasma ET1 may have no effect on platelet function.