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研究了土霉素对小麦种子发芽以及早期幼苗生长发育的生态毒性效应.结果表明,土霉素对小麦种子芽长及根长的抑制效应显著(p<0.01),而且它们之间具有良好的剂量-效应关系.根据线性回归方程得出土霉素对小麦种子芽长和根长的半抑制浓度为65.5 mg/L和34.7 mg/L.然而,土霉素对小麦种子的发芽率并没有显著的影响.研究还表明,0.15~2.4 mg/L土霉素暴露21 d后,小麦叶片中的叶绿素含量降低了35.6%~47.3%,叶片及根部的可溶性蛋白含量也均呈显著下降趋势.暴露7 d后,0.15~2.4 mg/L土霉素对小麦叶片和根部SOD与POD活性的抑制效应不显著,但随着暴露时间的延长,土霉素对小麦的SOD与POD酶活性抑制率显著下降;2.4 mg/L土霉素暴露21 d后,小麦根部的SOD活性下降72.3%,说明土霉素对小麦幼苗体内的抗氧化系统具有破坏作用.该实验结果显示,低浓度土霉素长期暴露对小麦幼苗的生长发育具有不良的生态毒性效应.
The ecotoxicological effects of oxytetracycline on the germination of wheat seeds and the growth and development of early seedling were studied.The results showed that oxytetracycline significantly inhibited the shoot length and root length of wheat (p <0.01), and had good Dose-effect relationship.According to the linear regression equation, the half inhibitory concentrations of oxytetracycline to the length and root length of wheat seeds were 65.5 mg / L and 34.7 mg / L. However, oxytetracycline had no significant effect on the germination rate of wheat seeds The results also showed that the chlorophyll content in wheat leaves decreased 35.6% -47.3% after 21 days exposure to 0.15-2.4 mg / L oxytetracycline, and the soluble protein content in leaves and roots also decreased significantly. After 7 days, the inhibitory effect of 0.15-2.4 mg / L oxytetracycline on the activities of SOD and POD in wheat leaves and roots was insignificant, but with the prolongation of exposure time, the inhibitory rates of oxytetracycline to SOD and POD activity of wheat significantly increased Decreased; SOD activity of wheat roots decreased by 72.3% after exposed to 2.4 mg / L oxytetracycline for 21 d, indicating that oxytetracycline had a damaging effect on antioxidant system of wheat seedlings.The results showed that low concentrations of oxytetracycline long-term Exposed to the growth of wheat seedlings have Ecotoxicological effects of good.