论文部分内容阅读
目的 研究苯乙烯的DNA加合特性。方法 采用紫外光谱移动法研究苯乙烯 7,8 氧化物 (SO)、苯乙烯、苯乙醇酸 (MA)、苯乙醛酸 (PGA)、苯乙烯巯基尿酸 (UMA)和DNA的加合反应 ;以3 2 P后标记法研究SO DNA加合物 ;以气相色谱 质谱、核磁共振研究SO DNA加合物的结构。结果 苯乙烯、MA、PGA和UMA不与DNA发生加合反应 ;SO分别在DNA脱氧鸟苷碱基上的O6位、N2 位形成 6种加合物。结论 苯乙烯进入机体后 ,通过其活性中间代谢物SO与DNA起加合作用 ,SO攻击DNA脱氧鸟苷碱基上的O6位、N2 位形成加合物 ,如果在细胞复制前所形成的DNA加合物没有被修复或者被错误修复的话 ,就有可能导致基因突变 ,产生化学损伤。苯乙烯的其他代谢物未见此效应。
Objective To study the DNA adduct properties of styrene. Methods The adduct of styrene, 7,8 oxide (SO), styrene, MA, PGA, UMA with DNA was studied by UV spectral shift method. The SO 2 adducts were studied by 3 2 P post-labeling method. The structure of SO DNA adducts was studied by gas chromatography-mass spectrometry and nuclear magnetic resonance. Results Styrene, MA, PGA and UMA did not undergo adduct reaction with DNA. SO formed 6 adducts respectively at O6 and N2 of DNA deoxyguanosine. Conclusion After styrene enters the body, SO and DNA play an important role through its active intermediate metabolite. SO attack the O6 position on the DNA deoxyguanosine base and form an adduct at the N2 position. If the DNA formed before cell replication Adducts that have not been repaired or are mistakenly repaired may cause mutations and chemical damage. Other metabolites of styrene did not show this effect.