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目的:探讨山楂叶总黄酮(TFHL)对非酒精性脂肪性肝病(NAFLD)模型大鼠的治疗作用及其机制。方法:将SD大鼠随机分为正常组,模型组,TFHL高、中、低剂量组,共5组,高脂膳食建立NAFLD大鼠模型。HE染色、油红O染色观察肝脏病理变化;生化仪检测血清血脂等;RT-PCR法检测肝组织FXR、PPAR-α、PGC1α、CYP7A1等的mRNA表达。Western Blot检测肝脏上述各项基因的蛋白表达。结果:与模型组比较,应用TFHL干预后,肝组织TG、CHOL含量显著降低,同时能增强肝脏FXR、PPAR-α、PGC1αmRNA和蛋白的表达,差异有统计学意义(P<0.05,P<0.01)。结论:TFHL可调节NAFLD大鼠肝脏的脂质代谢,改善炎性反应状态,其机制可能与调控FXR相关基因有关。
Objective: To investigate the therapeutic effect of hawthorn leaf flavonoids (TFHL) on nonalcoholic fatty liver disease (NAFLD) in rats and its mechanism. Methods: SD rats were randomly divided into normal group, model group, TFHL high, medium and low dose groups, a total of 5 groups, high fat diet to establish NAFLD rat model. HE staining and oil red O staining were used to observe the pathological changes of liver. Serum lipids were detected by biochemical analyzer. The mRNA expressions of FXR, PPAR-α, PGC1α and CYP7A1 were detected by RT-PCR. Western Blot detection of liver gene expression of the above genes. Results: Compared with the model group, the levels of TG and CHOL in liver tissue were significantly decreased after intervention with TFHL, and the mRNA and protein expressions of FXR, PPAR-α and PGC1α in liver increased at the same time (P <0.05, P <0.01) ). CONCLUSION: TFHL can regulate the lipid metabolism in the liver of NAFLD rats and improve the inflammatory response, which may be related to the regulation of FXR-related genes.