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目的开展肠道病毒71型(EV71)基因变异监测,为EV71型传染病防治提供依据。方法对福建省2013—2014年手足口监测标本进行病毒分离和鉴定。选取34株(每年各17株)各地市具有代表性EV71型分离株进行VP1基因测序,对所得序列进行比对,构建进化树,并分析氨基酸变异情况。结果 34株EV71型分离株均为C4a亚型,VP1区的核苷酸和氨基酸同源性分别为93.3%~99.8%和98.3%~100%,2013与2014年毒株VP1基因未见明显变异。分离株的氨基酸同源性比较,共有22株在6个位点的氨基酸发生突变。结论福建地区2013—2014年流行的EV71毒株基因型仍为C4a亚型,但序列进化和氨基酸变异分析提示,福建省存在不同的EV71传播链,应持续给予关注。
Objective To carry out the surveillance of the variation of EV71 gene in enteroviruses and provide evidence for the prevention and treatment of EV71-type infectious diseases. Methods The virus was isolated and identified from hand-foot-mouth surveillance samples from 2013 to 2014 in Fujian province. A total of 34 strains (17 strains each year) were selected to isolate VP1 gene from representative EV71 isolates in different provinces and cities. The sequences were compared and the phylogenetic tree was constructed and analyzed for amino acid variation. Results The 34 EV71 isolates were all C4a subtypes. The nucleotide and amino acid homology of VP1 was 93.3% -99.8% and 98.3% -100%, respectively. There was no significant variation in VP1 gene between 2013 and 2014 . Amino acid homology of the isolates, a total of 22 strains in the six amino acid mutations. Conclusion The genotypes of EV71 strains in Fujian province between 2013 and 2014 are still C4a subtypes. However, sequence evolution and amino acid variation analysis suggest that there are different EV71 transmission chains in Fujian Province and should be given continuous attention.