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将小菜蛾抗性品系与敏感品系杂交,F1代用能杀死全部敏感个体和部分杂合个体的剂量进行选择;存活个体与敏感品系回交,其后代再用上述适量药剂进行选择;存活个体与敏感品系回交。通过12代回交,建立了抗杀虫双和抗杀螟丹近等基因系,并对其多功能氧化酶环氧化活性和生物学特性进行了比较研究。结果显示:(1)抗杀虫双和抗杀螟丹小菜蛾近等基因系的艾氏剂环氧化活性均高于敏感品系,分别为1.56倍和1.97倍;(2)在回交后代中,抗性水平逐代降低。小菜蛾对杀虫双和杀螟丹的抗性并未付出明显的生物学代价。
Hybrids of the diamondback moth resistant lines and susceptible lines were used to select the F1 generation that could kill all sensitive individuals and some heterozygous individuals; the surviving individuals were backcrossed with the susceptible lines and their offspring were selected with the above appropriate amount of medicaments; Sensitive lines back to. Through the 12 generations of backcrossing, the near isogenic lines of insecticidal and resistance to Cartap were established, and their epoxidation activities and biological characteristics of polyfunctional oxidase were compared. The results showed that: (1) The epirubicidal epoxidation activities of anti-insecticidal and antifungal insects against diamondback moth (Plutella xylostella) were all higher than those of susceptible strains (1.56 and 1.97, respectively); (2) In the backcross progeny, the level of resistance decreased from generation to generation. Plutella xylostella insecticide double resistance and resistance to Cartap did not pay a significant biological price.