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目的:首次研究建立升麻提取物指纹图谱。方法:采用酚酸类和三萜皂苷类指纹图谱相结合的方式,以HypersilBDS C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-0.1%磷酸水为流动相梯度洗脱,流速均为1.0 mL.min-1,柱温为30℃,测定波长分别为316,210 nm。结果:酚酸类指纹图谱以13个共有峰为评价指标,精密度和重复性中共有峰相对保留时间和相对峰面积RSD均小于3.0%,10批样品的相似度均大于0.9;三萜皂苷类指纹图谱以14个共有峰为评价指标,精密度和重复性中共有峰相对保留时间和相对峰面积RSD均小于4.0%,10批样品的相似度均大于0.9。结论:该方法全面、稳定、可靠,可以有效用于升麻提取物纯化物的质量控制,为升麻提取物与同属植物提取物的比较及药效学差异的分析提供一定的参考,也为升麻提取物的进一步开发利用奠定基础。
Objective: To study the establishment of fingerprints of cimicifuga extract for the first time. Methods: The chromatographic column of Hypersil BDS C18 (4.6 mm × 250 mm, 5 μm) and the mobile phase of acetonitrile -0.1% phosphoric acid were used as the mobile phase. The flow rate was 1.0 mL.min-1, the column temperature was 30 ℃, the determination wavelength was 316,210 nm. Results: The phenolic acid fingerprints were evaluated by 13 common peaks. The RSDs of relative peak area and relative peak area were less than 3.0% in both precision and repeatability. The similarity of ten batches of samples were all greater than 0.9. The triterpene saponins The fingerprints of 14 fingerprints were used as the evaluation index. The relative retention time and the relative peak area (RSD) of the common peaks in precision and repeatability were both less than 4.0%. The similarity of 10 batches of samples were all greater than 0.9. Conclusion: The method is comprehensive, stable and reliable, and can be used effectively for the quality control of purified cimicifuga extracts. It provides some references for the comparison of the extracts of cimicifugae and the same plant and the analysis of pharmacodynamic differences. Cimicifuga extract further development and utilization of laid the foundation.