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目的:探讨人早孕滋养细胞在蜕膜白细胞选择性募集中的可能机制。方法:分离纯化人早孕滋养细胞进行原代培养,制备滋养细胞条件培养液(CM),分离蜕膜白细胞进行Transwell趋化实验,流式细胞术分析CM对蜕膜各型免疫细胞的趋化效应。结果:4倍稀释的CM即显示对蜕膜白细胞的显著趋化活性(P<0.05),而且二者之间呈显著直线正相关性(P<0.01);4倍稀释的CM即显示对CD56+CD16-和CD56+CD16+NK细胞、单核细胞、T细胞的显著趋化活性(P<0.05),趋化效率随CM浓度增高而上升;2倍稀释的CM显示对γδT细胞的趋化效应(P<0.05);CM对NKT细胞不表现趋化效应;经CM趋化前后的蜕膜白细胞构成比例相似(P>0.05)。结论:人早孕滋养细胞可能分泌多种趋化因子和细胞因子,募集和黏附到达蜕膜的母体免疫细胞,缔造母胎免疫耐受。
Objective: To explore the possible mechanism of human gestational trophoblastic cells in the selective recruitment of decidual leukocytes. Methods: Primary human gestational trophoblast cells were isolated and purified for primary culture. Trophoblastic conditioned medium (CM) was prepared, and decidual white blood cells were isolated for transwell chemotaxis assay. The chemotactic effects of CM on various types of immune cells in decidua were analyzed by flow cytometry . Results: The four-fold dilutions of CM showed significant chemotactic activity on decidual white blood cells (P <0.05), and there was a significant positive correlation between the two (P <0.01) + CD16- and CD56 + CD16 + NK cells, monocytes and T cells (P <0.05). The chemotactic efficiency increased with the increase of CM concentration. The two-fold dilution of CM showed chemotaxis to γδT cells (P <0.05); CM did not show chemotactic effect on NKT cells; the proportion of decidual white blood cells before and after CM chemotaxis was similar (P> 0.05). CONCLUSION: Human gestational trophoblasts may secrete a variety of chemokines and cytokines, recruiting and adhering to maternal immune cells that reach the decidua to create maternal-fetal immune tolerance.