Objective:In this study,we investigated the composition of the intestinal microflora associated with Yang-deficiency and the function-specific members of this microflora.Method:Subjects with normal and Yang-deficient constitutions were recruited in Beijing,with 30 subjects in each group.Illumina high-throughput sequencing was used to sequence the DNA of their fecal bacteria in the 16S rRNA V3-V4 region.The species abundance and distribution of the intestinal microflora in each specimen were determined with a statistical analysis of these sequences.We identified an underlying taxonomic trend with nonparametric PCoA and other statistical techniques.Results:(1) Subjects with Yang-deficiency displayed more uneven abundances of the taxa in their intestinal microflora than the subjects with normal Yang;(2) several differences were observed in the proportions of certain bacterial groups between the Yang-deficient and control groups;and (3) the fallowing bacteria differed significantly between the two groups:(i) Brevundimonas,Leuconostoc,Turicibacter,Defluviitaleaceae incertae sedis,Rothia,and Butyricimonas were slightly higher in the Yang-deficient subjects,whereas (ii)Megasphaera,Ruminococcus,and Lachnospira were higher in the normal subjects than in the Yang-deficient subjects.