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目的检测分析鲍氏不动杆菌的耐药性、全面了解多药耐药鲍氏不动杆菌氨基糖苷类耐药机制。方法药敏试验为K-B法、基因检测采用PCR法对20株多药耐药鲍氏不动杆菌(MDRAB)进行了7种氨基糖苷类修饰酶和2种16SrRNA甲基化酶基因检测。结果 20株鲍氏不动杆菌耐药率除亚胺培南外,对其余药物的耐药率均>95.0%,共有aac(3)-Ⅰ、aac(6′)-Ⅰb、ant(3″)-Ⅰ、aph(3′)-Ⅰ4种氨基糖苷类修饰酶基因阳性,阳性率分别为75.0%、90.0%、95.0%、65.0%,并检出armA 16SrRNA甲基化酶基因,阳性率为55.5%。结论该组MDRAB多种氨基糖苷类药物耐药与同时存在产氨基糖苷类修饰酶、产16SrRNA甲基化酶有关。
Objective To detect and analyze the drug resistance of Acinetobacter baumannii, to fully understand the mechanism of multidrug-resistant Acinetobacter baumannii aminoglycoside resistance. Methods The drug sensitivity test was K-B method. Seventeen aminoglycoside-modifying enzymes and two 16SrRNA methylase genes were detected in 20 multidrug-resistant Acinetobacter baumannii (MDRAB) strains by PCR. Results The drug resistance rates of 20 strains of Acinetobacter baumannii except imipenem were> 95.0%, the total drug resistance rates were aac (3) -Ⅰ, aac (6 ’) - Ⅰb, ant ) -Ⅰ, aph (3 ’) - Ⅰ were detected. The positive rates of armA 16SrRNA methylase gene were 75.0%, 90.0%, 95.0% and 65.0% 55.5% .Conclusion MDRAB multi-aminoglycoside resistance in this group is related to simultaneous production of aminoglycoside-modifying enzymes and production of 16SrRNA methylase.