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为了了解来自八倍体小滨麦与普通小麦杂种后代的小滨麦种质系山农0096的染色体构型和抗锈性,在农艺性状综合评价的基础上,对该系进行了细胞学和RAPD鉴定。结果表明,山农0096对条锈病免疫,其根尖细胞染色体数为2n=42,花粉母细胞减数分裂中期 (PMCMI)染色体构型为2n=21 ;与小麦亲本的杂种F1PMCMI染色体构型平均值为2n=19 +1 ;100个多聚体核苷酸随机引物中,有3个引物在山农0096中扩增出滨麦草的特异DNA带,它们分别记为S241250、S38750、S42640,初步确定山农0096是一个小滨麦易位系。
In order to understand the chromosome configuration and rust resistance of the germplasm Shannong 0096 from ecotypes of ecotypes of ecotypes of ecotypes and ecotypes of ecotypes, RAPD identification. The results showed that Shannong 0096 was resistant to stripe rust, the chromosome number of root tip cells was 2n = 42, and the chromosome configuration of pollen mother cells was 2n = 21 in metaphase metaphase The value of 2n = 19 +1; 100 polymer nucleotide random primers, three primers in Shannon 0096 amplified specific DNA bands of barley grass, which were recorded as S241250, S38750, S42640, preliminary Determine Shannon 0096 is a small wheat translocation line.