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目的构建７ｑ３２区域鼻咽癌细胞和组织及原代培养人正常鼻咽上皮细胞的部分基因表达图谱。方法通过差异ＲＴ－ＰＣＲ和Ｎｏｒｔｈｅｒｎ杂交的方法检测定位于７ｑ３２区域的２０个ＥＳＴ在鼻咽癌细胞和鼻咽癌组织及原代培养人正常鼻咽上皮细胞ｍＲＮＡ的表达水平。结果８个ＥＳＴ在鼻咽癌细胞ＨＮＥ１和原代培养人正常鼻咽上皮细胞中表达量较一致，７个ＥＳＴ在两种细胞中均无表达，３个ＥＳＴ（Ｗ７２６８８、Ｈ１９８３０、ＡＡ１３０６３０）在鼻咽癌细胞株中表达上调，而２个ＥＳＴ（ＡＡ０７０４３７、Ｈ９０８８２）在原代培养人鼻咽上皮细胞中表达上调。在１３例鼻咽癌活检组织中３０．７％（４／１３）的ＡＡ０７０４３７表达下调，７７％（１０／１３）的Ｗ７２６８８和７７％（１０／１３）的Ｈ１９８３０表达上升。结论构建了７ｑ３２区域鼻咽癌细胞和组织及原代培养人正常鼻咽上皮细胞部分基因表达图谱，并初步认为Ａ０７０４３７的表达下调和Ｗ７２６８８、Ｈ１９８３０的过表达与鼻咽癌的发生有关。
Objective To construct partial gene expression patterns of nasopharyngeal carcinoma cells and tissues in 7q32 region and normal human nasopharyngeal epithelial cells in primary culture. Methods The mRNA expression of 20 ESTs located in the 7q32 region of nasopharyngeal carcinoma, nasopharyngeal carcinoma and primary cultured normal human nasopharyngeal epithelial cells was detected by RT-PCR and Northern blot. Results Eight ESTs were expressed in normal nasopharyngeal epithelial cells of HNE1 and primary cultured human nasopharyngeal carcinoma cells. The seven ESTs were not expressed in the two cell lines. Three ESTs (W72688, H19830, AA130630) However, two ESTs (AA070437, H90882) were up-regulated in primary cultured human nasopharyngeal epithelial cells. AA070437 expression was down-regulated in 30.7% (4/13) of 13 nasopharyngeal carcinoma biopsies, with 77% (10/13) of W72688 and 77% (10/13) of H19830 up-regulated. Conclusion The partial gene expression profiles of nasopharyngeal carcinoma cells and tissues in 7q32 region and normal human nasopharyngeal epithelial cells in primary culture were constructed. The down-regulation of A070437 and overexpression of W72688 and H19830 were associated with the occurrence of nasopharyngeal carcinoma.