目的观察ＨＳＰ２７、ＨＳＰ７０与多种瘤抑制基因（ＭＴＳ１）、ｋｒａｓ基因及其蛋白产物在职业性肺癌（ｏｃｃｕｐａｔｉｏｎａｌｌｕｎｇｃａｎｃｅｒ，ＯＬＣ）中的联合表达的情况。方法采用免疫组化和原位分子杂交技术。结果ＯＬＣ中的ＨＳＰｓ表达较正常肺组织高；ＯＬＣ中的ＨＳＰｓ与ｋｒａｓ都有基因扩增和蛋白产物的过表达；ＭＴＳ１的表达与ＨＳＰｓ、ｋｒａｓ的表达呈负相关。结论ＭＴＳ１表达的变化可能是ＯＬＣ癌变的重要分子学改变；ＨＳＰｓ与ｋｒａｓ在ＯＬＣ的癌变过程中有协同作用；ＯＬＣ中以上３种基因在基因水平及蛋白产物表达上的改变基本一致。 Objective To observe the combined expression of HSP27, HSP70 and multiple tumor suppressor genes (MTS1) and kras genes and their protein products in occupational lung cancer (OLC). Methods Immunohistochemistry and in situ molecular hybridization techniques were used. Results The expression of HSPs in OLC was higher than that in normal lung tissues. Both HSPs and kras in OLC had gene amplification and overexpression of protein products. The expression of MTS1 was negatively correlated with the expression of HSPs and kras. Conclusion The change of MTS1 expression may be an important molecular change of OLC cancer; HSPs and kras have a synergistic effect in the carcinogenesis of OLC; the changes of the above three genes in OLC are basically the same at the gene level and protein product expression.