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目的探讨以微乳超声法同时提取鹿角灵芝中灵芝酸和灵芝多糖的最佳条件。方法采用星点设计-效应面法,以物料溶剂比(X1)、超声提取时间(X2)和超声功率(X3)作为考察因素,以灵芝酸A、灵芝酸C2、灵芝多糖含量和提取物得率为考察指标,并对考察因素和考察指标总评归一值进行多元线性回归和二项式拟合,以效应面优选最佳的微乳超声提取工艺,并做验证试验。结果微乳超声提取鹿角灵芝的最佳条件为物料溶剂比1∶18、超声提取时间59 min、超声功率513 W,此条件下灵芝酸A、灵芝酸C2、灵芝多糖含量和提取物得率分别为0.647 8,0.108 5,11.20 mg·g?1和34.28%。结论微乳超声可同时提取鹿角灵芝中脂溶性和水溶性成分,并且节能、省时、操作简便,为工业化提取鹿角灵芝提供了新途径。
Objective To investigate the optimal conditions for the simultaneous extraction of Ganoderma lucidum and Ganoderma lucidum polysaccharides from Ganoderma lucidum by microemulsion ultrasonic method. Methods The star point design-response surface method was used to investigate the effects of ganoderic acid A, ganoderic acid C2, ganoderma lucidum polysaccharide content and extract on the solvent ratio (X1), ultrasonic extraction time (X2) and ultrasonic power (X3) Rate as the index of investigation, and multivariate linear regression and binomial fitting were carried out on the regression coefficients of the investigation factors and the evaluation indexes. The optimal microemulsion ultrasonic extraction process was optimized by the effect surface and the validation test was done. Results The optimum conditions for ultrasonic extraction of Ganoderma lucidum by microemulsion were solvent ratio 1:18, ultrasonic extraction time 59 min, ultrasonic power 513 W, the contents of ganoderic acid A, ganoderic acid C2, ganoderma lucidum polysaccharide and the yield of extract were respectively 0.647 8, 0.108 5, 11.20 mg · g -1, and 34.28%. Conclusion The microemulsion ultrasound can extract fat-soluble and water-soluble components of Ganoderma lucidum at the same time, save energy, save time, and be easy to operate. It provides a new way for the industrialization of Ganoderma lucidum.