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自从1954年du Vigneaud等合成了催产素后,Boissonnas等(1955)、Rudinger等(1956)、Velluz等(1956)相继报导了催产素的合成方法。在合成肽链时,前三者主要采用了迭氮化合物、混合酸酐和亚磷衍生物等方法而后者则应用了较新的二环己基碳二亚酰胺方法。在合成步骤上,du Vigneaud等由三肽异亮氨酰谷氨酰胺酰门冬酰胺、四肽半胱氨酰脯氨酰亮氨酰甘氨酰胺、和二肽半胱氨酰酪氨酸依次缩合成七肽、九肽,再经金属钠在液态氨中处理和进一步氧化而成具有生理活性的八肽——催产素。
Since the synthesis of oxytocin by du Vigneaud et al. In 1954, Boissonnas et al. (1955), Rudinger et al. (1956) and Velluz et al. (1956) reported on the synthesis of oxytocin in succession. In the synthesis of peptide chains, the first three methods used mainly azide compounds, mixed anhydride and phosphorous derivatives and other methods while the latter application of the new dicyclohexylcarbodiimide method. At the synthetic step, du Vigneaud et al. Followed by tripeptide isoleucyl glutaminyl asparagine, tetrapeptide cysteinyl prolyl leucylglycinamide, and dipeptide cysteinyl tyrosine Condensed into heptapeptide, nonapeptide, and then sodium metal in liquid ammonia treatment and further oxidation into a physiologically active octapeptide - oxytocin.