采用离体叶片接种致病性测定、形态学观察和分子鉴定方法对非洲菊叶斑病病原进行鉴定。从北京某花卉基地采集非洲菊叶斑病样,分离获得22个真菌分离物,分别记作GL1-GL22。经过致病性试验证实,分离物GL14和GL22为非洲菊叶斑病病原菌。经形态学鉴定,GL14为细极链格孢Alternaria tenuissima,GL22为链格孢Alternaria alternata。采用真菌通用引物对GL14和GL22 rDNA的ITS区进行PCR扩增,并将测序结果在GenBank中进行同源性比对分析,分子鉴定与形态学鉴定结果一致。 Pathogenicity test, morphological observation and molecular identification methods were used to identify the pathogen of gerbera leaf spot. Gerbera leaf spot samples were collected from a flower base in Beijing and 22 fungal isolates were isolated and recorded as GL1-GL22 respectively. After pathogenicity test confirmed that isolates GL14 and GL22 gerbera leaf spot pathogens. Morphological identification showed that GL14 was Alternaria tenuissima and GL22 was Alternaria alternata. The universal fungi primers were used to amplify the ITS region of GL14 and GL22 rDNA, and the results of sequencing were compared in GenBank. The results of molecular identification and morphological identification were consistent.