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实验采用标准玻璃微电极细胞内记录技术记录心肌细胞动作电位（action potential,AP）、肌力换能器记录心肌收缩力（force contraction,Fc）,研究乙酰胆碱（acetylcholine,ACh）对离体豚鼠心房肌、心室肌的作用。结果表明,10μmol/L ACh可缩短心房肌、心室肌动作电位的时程（action potential duration,APD）。心房肌APD在给药前后分别为208.57±36.05ms及101.78±14.41ms（n=6,P<0.01）,心室肌APD在给药前后分别为286.73±36.11ms及265.16±30.06 ms（n=6,P<0.01）。心房肌动作电位的幅度（action potential amplitude,APA）也降低,给药前后分别为88.00±9.35 mV及62.62±20.50 mV（n=6,P<0.01）,而心室肌APA无明显变化。ACh还降低心房肌、心室肌的收缩力,心房肌、心室肌Fc的抑制率分别为100％（n=6,P<0.01）和37.57±2.58％（n=6,P<0.01）。ACh对心房肌、心室肌APD和Fc的抑制作用在一定范围内（1nmol/L～100μmol/L）随ACh浓度的增高而增强。用Scott法求出ACh对心房肌、心室肌APD缩短作用的KD值,分别为0.275和0.575μmol/L,对Fc抑制作用的KD值分别为0.135和0.676μmol/L。各浓度下ACh对心房肌效应与心室肌效应作组间t检验,从10nmol/L到0.1mmol/L均有显著的统计学差异。此外,10μmol/L阿托品及20mmol/L
In this study, standard glass microelectrode intracellular recording technique was used to record the action potential (AP) of cardiomyocytes, and the force contraction (Fc) was recorded by muscle transducer. The effects of acetylcholine (ACh) Muscle, ventricular function. The results showed that 10μmol / L ACh could shorten the action potential duration (APD) of atrial myocardium and ventricular muscle. The atrial APD was 208.57 ± 36.05ms and 101.78 ± 14.41ms respectively before and after administration (n = 6, P <0.01), APD of ventricular muscle were 286.73 ± 36.11 ms and 265.16 ± 30.06 ms before and after administration (n = 6 , P <0.01). The action potential amplitude (APA) of atrial myocardium was also decreased before and after the treatment, with 88.00 ± 9.35 mV and 62.62 ± 20.50 mV before and after administration (n = 6, P <0.01), while the ventricular APA did not change significantly. ACh also reduced atrial and ventricular contractility, and the inhibitory rates of atrial and ventricular myocardium Fc were 100% (n = 6, P <0.01) and 37.57 ± 2.58% (n = 6, P <0.01), respectively. The inhibitory effect of ACh on atrial myocardium, ventricular APD and Fc in a certain range (1nmol / L ~ 100μmol / L) increased with the increase of ACh concentration. The KD values of shortening ACh on atrial and ventricular muscles were 0.275 and 0.575μmol / L, respectively, and the KD values of inhibition on Fc were 0.135 and 0.676μmol / L, respectively. ACh on each concentration of atrial muscle effect and ventricular muscle effect for the t-test between groups, from 10nmol / L to 0.1mmol / L were statistically significant differences. In addition, 10μmol / L atropine and 20mmol / L