提出了一种用于求解非扫描式光学SPR共振反射光谱信号的共振像素位点的最小二乘法多项式拟合方法,最大限度地提高信噪比.描述了滑动平均值滤波、多项式数据拟合方法的原理和共振反射光谱最低点的质心算法.采用体积分数为13%,14%,16%,17%,18%,19%的乙醇溶液进行试验验证.结果表明,多项式拟合算法对光功率变化等环境因素所引起的噪声具有很好的抑制作用,相关系数为-0.985 2,灵敏度为959.60. A least squares polynomial fitting method for solving the resonance pixel position of unscanned optical SPR resonance reflectance signal is proposed to maximize the signal to noise ratio.The paper describes the methods of sliding average filtering and polynomial data fitting And the centroid algorithm of the lowest point of the resonance reflectance spectrum.Experimental results show that the volume fraction of 13%, 14%, 16%, 17%, 18%, 19% Change and other environmental factors caused by the noise has a good inhibitory effect, the correlation coefficient of -0.985 2, the sensitivity of 959.60.