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金、银纳米粒子因其独特的光线、电学、磁性和结构特性而被广泛应用于光电存储、生物医疗、化工环境等领域。和传统的制备方法相比,生物制备方法具有良好的生物相容性、反应条件温和、绿色且可持续发展等优点。利用大肠杆菌制备了金、银纳米粒子,采用紫外-可见光谱研究了反应条件,透射电镜与能谱表征了材料的形貌和成分,并通过红外光谱探究大肠杆菌还原金银的机制。结果表明:反应时间60min、贵金属前驱体浓度为1.0g/L时,大肠杆菌还原所得的金、银纳米粒子平均粒径分别为10.82nm和14.71nm,且大小比较均一。红外分析结果表明大肠杆菌在还原贵金属前驱体时,多羟基化合物、蛋白质类物质等起还原作用。
Gold and silver nanoparticles are widely used in optoelectronic storage, biomedicine and chemical environment for their unique light, electricity, magnetism and structural properties. Compared with the traditional preparation method, the biological preparation method has the advantages of good biocompatibility, mild reaction conditions, green and sustainable development. The gold and silver nanoparticles were prepared by using Escherichia coli. The morphology and composition of the material were characterized by UV-Vis spectroscopy, transmission electron microscopy and energy dispersive spectroscopy. The mechanism of Escherichia coli reducing gold and silver was also studied by infrared spectroscopy. The results showed that when the reaction time was 60 min and the concentration of noble metal precursor was 1.0 g / L, the average diameters of the gold and silver nanoparticles obtained by the reduction of E. coli were 10.82 nm and 14.71 nm, respectively, and the sizes were relatively uniform. Infrared analysis showed that Escherichia coli in the reduction of precious metal precursors, polyols, proteins and other substances play a role in reducing.