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目的探讨褪黑素(Mel)在匹罗卡品(PILO)致癫癎犬鼠模型中的抗癫癎作用机制。方法将45只Wistar大鼠按癫癎持续状态(SE)后6 h,14、28天分为PILO组(1 5只),PILO+Mel组(15只)和对照组(15只),采用PILO诱导大鼠慢性颞叶癫癎模型,用5溴-2-脱氧尿嘧啶核苷标记增殖细胞,Timms染色评价苔藓纤维发芽(MFS)等技术,动态观察MeI对癫癎大鼠海马神经发生和MFS的影响及其与反复自发性癫癎发作(SRS)发生的关系。结果与对照组比较,PILO组大鼠SE后6 h,14、28天细胞数明显增加,差异有统计学意义(P<0.01);与PILO组比较,PILO+Mel组大鼠在SE后6 h,14、28天,细胞数量明显减少(P<0.05),28天SRS数量明显减少,差异有统计学意义(P<0.05)。与PILO+Mel组比较,PILO组大鼠SE后14天,Timms染色密度开始增强,28天密度明显增强,差异有统计学意义(P<0.05)。结论Mel对SRS的预防作用可能与其对癫癎诱导的神经发生和MFS的抑制作用有关。
Objective To investigate the anti-epileptic mechanism of melatonin (Mel) in pilocarpine-induced epileptic canine model rats. Methods 45 Wistar rats were divided into PILO group (15), PILO + Mel group (15) and control group (15) 6 h, 14 and 28 days after epileptic seizure (SE) The model of chronic temporal lobe epilepsy was induced by PILO in rats. The proliferative cells were labeled with 5-bromo-2-deoxyuridine and the mossy fiber sprouting (MFS) was assessed by Timms staining. The effects of MeI on neurogenesis in hippocampus of rats with epilepsy and MFS and its relationship with recurrent spontaneous epileptic seizures (SRS). Results Compared with the control group, the number of cells in PILO group increased significantly at 6 h, 14 and 28 days after SE (P <0.01), compared with PILO group (P <0.05). The number of SRS significantly decreased on the 28th day, the difference was statistically significant (P <0.05). Compared with PILO + Mel group, the Timms staining density of PILO group began to increase 14 days after SE, and the density of 28 days significantly increased (P <0.05). Conclusion The preventive effect of Mel on SRS may be related to the inhibition of epilepsy-induced neurogenesis and MFS.