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目的比较慢性苯中毒患者与健康人的血清蛋白质组,筛选差异表达蛋白质,为阐明其发病机制和寻找生物标志物提供靶标。方法免疫层析柱去除14种高丰度血清蛋白质后,应用8标iTRAQ技术结合2D LC-MS/MS分析并鉴定慢性苯中毒病例与健康人血清的差异表达蛋白质。结果对9例慢性苯中毒患者和9名健康人的血清蛋白样品的iTRAQ标记,分析并成功鉴定159种蛋白质,其中差异表达有统计学意义(P<0.05)的蛋白质有纤维蛋白溶酶原(PLG)、载脂蛋白B100(APOB100)、血小板碱性蛋白(PBP)。这些差异表达蛋白质具有结合、催化、酶调节和转运活性等分子功能,参与的生物过程包括免疫、代谢、应激、转运和凋亡等。结论 PLG、APOB100和PBP可能作为慢性苯中毒发病机制研究的候选靶标。
Objective To compare the serum proteomes between patients with chronic benzene poisoning and healthy people and screen the differentially expressed proteins for the purpose of elucidating the pathogenesis and searching for biomarkers. Methods After removing 14 kinds of high-abundance serum proteins by immunochromatography, the 8-labeled iTRAQ technology combined with 2D LC-MS / MS was used to analyze and identify differentially expressed proteins in serum of patients with chronic benzene poisoning and healthy people. Results A total of 159 proteins were identified and successfully identified by iTRAQ in serum samples of 9 patients with chronic benzene poisoning and 9 healthy individuals. Among them, the proteins with statistically significant difference (P <0.05) were plasminogen PLG), apolipoprotein B100 (APOB100), platelet basic protein (PBP). These differentially expressed proteins have molecular functions such as binding, catalysis, enzyme regulation and transport activity, and involved biological processes including immunity, metabolism, stress, transport and apoptosis. Conclusion PLG, APOB100 and PBP may serve as candidate targets for the study of pathogenesis of chronic benzene poisoning.