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目的：观察低分子ＲＮＡ 及５－ Ｆｕ 在白血病细胞系Ｋ５６２ 细胞中的作用。方法：在Ｋ５６２细胞培养中加低分子ＲＮＡ（ 实验组１） 、５ － Ｆｕ（ 实验组２） 、低分子ＲＮＡ 和５ － Ｆｕ（ 实验组３） 及Ｐ－Ｂ－Ｓ（ 对照组），观察集落细胞数，死、活细胞计数及细胞蛋白含量。结果：集落细胞数对照组为１４２ 个，实验组１ 为７０ 个，抑制率为５０－７ ％ ，实验组２ 为７１ 个，抑制率为５０－５％ ，实验组３ 为４１个，抑制率为７１－１ ％ ；活、死细胞实验组１ 为４２ 个、１１ 个，实验组２ 为４３ 、５ 个，实验组３ 为２７ 个、１６ 个；细胞蛋白含量实验组１ 为１－６１０ ，实验组２ 为１－６２２ ，实验组３ 为０－８７５ ，占７２－７ ％ 。结论：低分子ＲＮＡ能对白血病细胞系Ｋ５６２ 细胞的增殖有明显抑制作用，且能增强５ － Ｆｕ 抑制肿瘤细胞的增殖效果。
Objective: To observe the role of low molecular RNA and 5-Fu in leukemia cell line K562 cells. METHODS: Low-molecular RNA (experiment 1), 5 - Fu (experiment 2), low-molecular RNA, 5 - Fu (experiment 3), and P-B-S (control) were added to K562 cell culture. Number of colony cells, dead, viable cell counts, and cellular protein content. Results: The number of colony cells in the control group was 142, the experimental group 1 was 70, the inhibition rate was 50-7 %, the experimental group 2 was 71, the inhibition rate was 50-5%, the experimental group 3 was 41 and the inhibition rate 71-11%; viable and dead cell experimental group 1 was 42 and 11; experimental group 2 was 43 and 5; experimental group 3 was 27 and 16; cell protein content experimental group 1 was 1-610. The experimental group 2 was 1-622, and the experimental group 3 was 0-875, accounting for 72-7 %. Conclusion: Low-molecular RNA can significantly inhibit the proliferation of leukemia cell line K562 cells, and can enhance the effect of 5-Fu on tumor cell proliferation.