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目的探讨促红细胞生成素(EPO)对人骨髓间充质干细胞(MSCs)向血管内皮细胞诱导分化的影响。方法从正常人骨髓中分离获取MSCs,体外培养扩增、纯化后,分4组对其进行诱导分化:(1)EPO组;(2)碱性成纤维细胞生长因子(bFGF)+血管内皮细胞生长因子(VEGF)组;(3)EPO+bFGF+VEGF组;(4)DMEM培养液对照组。诱导前后行流式细胞仪检测细胞表面标记CD29、CD31、CD34、CD44,免疫细胞化学鉴定vWF抗体。结果 EPO组、bFGF+VEGF组及EPO+bFGF+VEGF组诱导MSCs后的细胞CD31、CD34表达率升高,CD29、CD44表达率降低,vWF抗体阳性。结论 EPO可以诱导MSCs向血管内皮细胞分化,但联合bFGF、VEGF并不能进一步促进MSCs向血管内皮细胞转化。
Objective To investigate the effect of erythropoietin (EPO) on differentiation of human bone marrow mesenchymal stem cells (MSCs) into vascular endothelial cells. Methods MSCs were isolated from normal human bone marrow and cultured in vitro. After being purified, the cells were divided into 4 groups to induce differentiation: (1) EPO group; (2) basic fibroblast growth factor (bFGF) + vascular endothelial cells Growth factor (VEGF) group; (3) EPO + bFGF + VEGF group; (4) DMEM culture medium control group. Cell surface markers CD29, CD31, CD34 and CD44 were detected by flow cytometry before and after induction and vWF antibodies were identified by immunocytochemistry. Results After the MSCs were induced by EPO, bFGF + VEGF and EPO + bFGF + VEGF, the expression of CD31 and CD34 increased, the expression of CD29 and CD44 decreased and the expression of vWF was positive. Conclusion EPO can induce the differentiation of MSCs into vascular endothelial cells. However, combination of bFGF and VEGF can not promote the transformation of MSCs into vascular endothelial cells.