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【目的】观察淫羊藿对纳米羟基磷灰石/胶原/聚乳酸(nHAC/PLA)在骨缺损修复过程中的成骨作用。【方法】制备16只兔双侧下颌骨10 mm×8 mm贯穿性缺损模型,随机分为中药淫羊藿组与对照组,2组左侧缺损不添加材料,右侧缺损处添加活性材料nHAC/PLA。术后2、4、8、12周分别处死动物,从大体、影像学、组织学方面进行观察,并计量新骨面积。【结果】影像学观察结果显示淫羊藿右侧组、对照右侧组、淫羊藿左侧组2、4、8、12周缺损区密度均高于对照左侧组。组织学观察可见淫羊藿右侧组、对照右侧组各时间点骨缺损区新骨形成均优于左侧组,新生骨及新生血管的量多。随着时间延长,淫羊藿右侧组、对照右侧组有大量骨样组织长入活性材料,骨成熟度增高,淫羊藿右侧组可见残留材料逐渐被活跃生长的骨组织包围并替代。分别与同侧对照组比较,淫羊藿左、右侧组新骨面积均显著升高,差异有统计学意义(P<0.05);同组左侧与右侧分别比较,差异均有统计学意义(P<0.05)。【结论】淫羊藿在骨缺损修复期间有良好的促进骨愈合的作用,nHAC/PLA活性材料具有良好的生物相容性、骨传导性及骨诱导活性,有望应用于临床修复骨缺损。
【Objective】 To observe the osteogenesis effect of Epimedium on nHAC / PLA in repairing bone defect. 【Methods】 A bilateral 10 mm × 8 mm penetrating defect model of mandible was made in 16 rabbits. The model was randomly divided into three groups: the epimedium herb group and the control group. No material was added to the left defect and the nHAC / PLA. The animals were sacrificed at 2, 4, 8 and 12 weeks after operation respectively, and the gross, radiological and histological changes were observed and the new bone area was measured. 【Results】 Imaging results showed that the densities of 2,4,8,12 weeks lesion in the left side of Epimedium right group, the control right side group and the Epimedium left side group were all higher than those in the control left side group. Histological observation shows Epimedium right group, right control group at each time point new bone formation in the defect area are better than the left group, the amount of new bone and neovascularization. With the extension of time, Epimedium right group, right control group, a large number of bone-like tissue grow into the active material, bone maturation increased, epimedium right group visible residual material gradually being surrounded by active growth of bone tissue and replace . Compared with the ipsilateral control group, the area of new bone in the left and right epimedium groups were significantly increased, the difference was statistically significant (P <0.05); on the left and right sides of the same group, the differences were statistically Significance (P <0.05). 【Conclusion】 Epimedium can promote bone healing during bone defect repair. The nHAC / PLA active material has good biocompatibility, osteoconductivity and osteoinductive activity, which is expected to be used clinically to repair bone defects.