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目的 :探讨人绒毛膜促性腺激素 -β反义寡脱氧核苷酸 (h CG-β AS-ODN)抑制 JAR绒癌细胞株 h CG的分泌。方法 :设计 h CG-β m RNA的 AS-ODN,作用于体外培养的 JAR绒癌细胞株 ,细胞浓度为 1× 1 0 5/个。ODN的浓度分别为 5 μmol/ L、1 0 μmol/ L、2 0 μmol/ L 与 JAR细胞共培养在96孔培养板中 ,2 4h、48h、72 h和 96 h,取上清测 h CG-β含量。结果 :1 0μmol/ L浓度的 h CG-βAS-ODN对 JAR细胞在体外作用 2 4h,h CG-β分泌呈下降趋势 ,48h下降达 5 7% ,此后 ,抑制作用略有减弱 ,而 2 0 μmol/ L的正义和随机的 ODN,分泌也受抑制。结论 :5～ 1 0 μmol/ L 的 AS-ODN明显抑制体外培养的 h CG分泌细胞 h CG蛋白的分泌 ,且该 AS-ODN有希望作为 h CG分泌抑制物应用。
OBJECTIVE: To investigate the inhibitory effect of h CG-β AS-ODN on h CG production in JAR choriocarcinoma cell line. Methods: The h CG-β m RNA AS-ODN was designed to act on JAR chondrocytes cultured in vitro at a cell concentration of 1 × 10 5 / cell. ODN were co-cultured with JAR cells at 5 μmol / L, 10 μmol / L and 20 μmol / L in 96-well plates for 24 h, 48 h, 72 h and 96 h, respectively. -β content. Results: h CG-βAS-ODN at a concentration of 10 μmol / L had an inhibitory effect on JAR cells in vitro for 24 h, h CG-β secretion decreased and reached 57% after 48 h. Secretion was also inhibited by both a sense and a random ODN of micromol / L. CONCLUSION: 5 ~ 10 μmol / L AS-ODN significantly inhibits h CG protein secretion in cultured h CG secreting cells in vitro, and AS-ODN is promising as h CG secretion inhibitor.