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目的:建立HPLC-ELSD法,采用正交试验优化黄芪甲苷的提取工艺。方法:采用HPLC-ELSD法,考察加水量、提取时间、提取次数3个主要因素对黄芪甲苷提取效率的影响,并应用MATLAB软件进行运算得出优选结果及各因素水平趋势图。结果:黄芪甲苷的最佳提取工艺为10倍水,提取3次,每次1h。结论:该提取工艺稳定可行,可用于藿芪温阳丸的质量控制。
OBJECTIVE: To establish HPLC-ELSD method and optimize the extraction process of Astragaloside IV by orthogonal experiment. Methods: HPLC-ELSD method was used to investigate the effect of three main factors on the extraction efficiency of Astragaloside Ⅳ, such as the amount of water, the extraction time and the number of extraction. The results of optimization and the horizontal trend of each factor were calculated by MATLAB software. Results: Astragaloside best extraction process is 10 times the water, extracted three times, each time 1h. Conclusion: The extraction process is stable and feasible and can be used for the quality control of Huoxiang Wenyang Wan.