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合成了介孔二氧化硅负载金纳米颗粒(Au-MSN),通过壳聚糖(CHIT)将Au-MSN固定到裸玻碳电极表面,采用自组装法将带巯基的血小板衍生生长因子(PDGF)核酸适体固定到Au-MSN修饰过的玻碳电极表面,制得PDGF核酸适体传感器.以亚甲基蓝作为电化学活性嵌入剂,通过检测核酸适体与目标分析物PDGF特异性结合前后亚甲基蓝电信号的变化,实现了对PDGF的定量检测.考察了缓冲溶液的pH、扫描速度及PDGF培育时间等条件对检测结果的影响.结果表明,在pH为7.6时,该传感器的检测范围为0.1pg/mL～1μg/mL,检出限为0.03 pg/mL.该传感器制作简单、成本低廉、灵敏度高且稳定性好.
Mesoporous silica supported gold nanoparticles (Au-MSN) were synthesized and Au-MSN was immobilized on the surface of bare glassy carbon electrode by chitosan (CHIT). Self-assembly method was used to separate the platelet-derived growth factor ) Aptamer was immobilized on the surface of Au-MSN modified glassy carbon electrode to prepare PDGF aptamer sensor.The methylene blue was used as an electrochemical active intercalator to detect the aptamer and target analyte PDGF specific binding before and after methylene blue And the change of signal, the quantitative detection of PDGF was realized.The influence of pH, scanning speed and incubation time of PDGF on the detection results was investigated.The results showed that the detection range of this sensor was 0.1pg / mL ~ 1μg / mL, the detection limit of 0.03 pg / mL.The sensor is simple, low cost, high sensitivity and good stability.