目的:建立HPLC法分离和测定尿石通丸中夏佛塔苷和橙皮苷的含量。方法:以Agilent Zobrax SB C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为乙腈-甲醇-0.4%甲酸溶液,梯度洗脱,检测波长为272 nm,流速为1.0 ml·mim-1,进样量:10μl。结果:夏佛塔苷在0.000 6~0.277 2 mg·ml-1范围内线性关系良好(r=1.000 0);橙皮苷在0.002 1~0.856 8 mg·ml-1范围内线性关系良好(r=1.000 0)。夏佛塔苷平均回收率为101.83%,RSD=0.27%,橙皮苷平均回收率为98.35%,RSD=0.41%(n=6)。结论:本方法可用于测定尿石通丸中夏佛塔苷和橙皮苷的含量。 OBJECTIVE: To establish an HPLC method for the determination of sumiflorin and hesperidin in urolitholithoid. Methods: The Agilent Zobrax SB C18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of acetonitrile-methanol-0.4% formic acid and eluted with a gradient of 272 nm at a flow rate of 1.0 ml · mim- 1, injection volume: 10μl. Results: There was a good linear relationship between the glycosides in the range of 0.0006 ~ 0.277 2 mg · ml-1 (r = 1.000 0) and the good linearity of hesperidin in the range of 0.002 1 ~ 0.856 8 mg · ml-1 = 1.000 0). The average recovery rate of summerostatidin was 101.83%, RSD = 0.27%. The average recovery rate of hesperidin was 98.35%, RSD = 0.41% (n = 6). Conclusion: This method can be used to determine the content of Xiaofutong glycosides and hesperidin.