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目的:通过胰腺病理学评分、血清淀粉酶和肝肾功能的变化,观察早期静脉应用鱼油脂肪乳剂(FO)对实验大鼠重症急性胰腺炎(SAP)炎症反应的影响,并探讨其可能的机制。方法:将大鼠随机分为正常组(NG,n=6)、鱼油治疗组(FOG,n=18)、大豆油(SOG,n=18)组和等渗盐水组(NSG,n=18)。建立大鼠SAP模型。建模120 min后,对FOG组、SOG组和NSG组进行相应干预。检测大鼠血清淀粉酶(AMS)、ALT、AST、尿素氮(BUN)、肌苷(Cr)、IL-1β和IL-10的水平,测定胰腺组织NF-κB的活性,胰腺组织行病理学评分,并比较各检测指标在不同组的差异。结果:①SAP大鼠血清AMS明显升高(正常值3倍以上),胰腺病理评分增高(2分及以上);FOG组6 h血清AMS低于SOG组和NSG组(P<0.05);FOG组2、6 h平均病理评分低于SOG组和NSG组(P<0.01)。②与NG组比,SAP大鼠血清ALT、AST、BUN、Cr值明显升高(P<0.01);FOG组血清ALT、BUN低于SOG组和NSG组(P<0.05)。③SAP大鼠胰腺组织NF-κB明显活化,FOG组胰腺组织活化NF-κB的A值低于SOG组和NSG组(P<0.05)。结论:①FO能降低SAP大鼠IL-1β的表达,增加IL-10的表达,有助于减轻SAP的过度炎症反应。②FO可能通过调节NF-κB的活性,影响IL-1β和IL-10的表达。③早期静脉应用FO能有效地减轻SAP的胰腺损伤,改善肝肾功能。
OBJECTIVE: To observe the effect of early application of fish oil emulsion (FO) on the inflammatory reaction of severe acute pancreatitis (SAP) in rats through the changes of pancreatic pathology score, serum amylase and liver and kidney function, and to explore its possible mechanism . Methods: The rats were randomly divided into normal group (NG, n = 6), fish oil treated group (FOG, n = 18), soybean oil (SOG, n = 18) group and isotonic saline group ). Establishment of rat SAP model. After 120 min of modeling, the corresponding interventions were performed on FOG, SOG and NSG groups. The levels of serum amylase (AMS), ALT, AST, BUN, Cr, IL-1β and IL-10 were measured and the activity of NF-κB in pancreatic tissue was determined. Score, and compare the different indicators in different groups. Results: ① The serum AMS in SAP rats was significantly higher than normal (3 times more than normal), and the pathological score of pancreatic increased (2 points or more); the serum AMS in FOG group was lower than that in SOG and NSG groups (P <0.05) The mean pathological score at 2,6 h was lower than that in SOG and NSG groups (P <0.01). ②Compared with NG group, serum ALT, AST, BUN and Cr in SAP group were significantly increased (P <0.01); ALT and BUN in FOG group were lower than those in SOG group and NSG group (P <0.05). (3) NF-κB activation in pancreas of SAP rats was significantly higher than that in SOG and NSG groups (P <0.05). Conclusion: FO can reduce the expression of IL-1β and increase the expression of IL-10 in SAP rats, which may help to reduce the excessive inflammatory reaction in SAP. ② FOX may affect the expression of IL-1β and IL-10 by regulating the activity of NF-κB. ③ early venous application of FO can effectively reduce SAP pancreatic injury, improve liver and kidney function.