论文部分内容阅读
目的探讨转化生长因子-β1在急、慢性特发性血小板减少性紫癜(ITP)患儿中的改变及其意义。方法2007年1月至12月华中科技大学同济医学院附属同济医院收集40例急性ITP(AITP)、13例慢性ITP(CITP)及31例非血液病儿童骨髓片,免疫组化法检测骨髓巨核细胞TGF-β1含量。结果AITP患儿的巨核细胞中的TGF-β1水平明显高于CITP及对照组(P=0.002)。而CITP组和对照组差异无统计学意义(P>0.05)。骨髓巨核细胞的平均灰度值与血小板数呈正相关性(r=0.264,P=0.016),即TGF-β1含量越高,血小板数越低。AITP平均灰度与幼巨核细胞比例呈负相关(r=-0.348,P=0.028),即AITP组TGF-β1含量越高幼巨核细胞比例越高。结论巨核细胞的TGF-β1在AITP发病中起到重要作用,而在CITP来自巨核细胞的TGF-β1可能没有参与血小板减少和巨核细胞成熟障碍的机制。
Objective To investigate the changes and clinical significance of transforming growth factor-β1 in children with acute and chronic idiopathic thrombocytopenic purpura (ITP). Methods From January 2007 to December 2007, 40 patients with acute ITP (AITP), 13 patients with chronic ITP (CITP) and 31 children with non-hematologic disease were collected from Tongji Hospital, Huazhong University of Science and Technology. Immunohistochemistry was used to detect bone marrow megakaryocytes Cell TGF-β1 content. Results The level of TGF-β1 in megakaryocytes of children with AITP was significantly higher than that of CITP and control (P = 0.002). There was no significant difference between CITP group and control group (P> 0.05). The average gray value of bone marrow megakaryocytes and platelet number was positively correlated (r = 0.264, P = 0.016), that is, the higher the content of TGF-β1, the lower the number of platelets. The mean gray level of AITP was negatively correlated with the ratio of young megakaryocytes (r = -0.348, P = 0.028). The higher the proportion of TGF-β1 in AITP was, the higher the proportion of young megakaryocytes was. Conclusion TGF-β1 in megakaryocytes plays an important role in the pathogenesis of AITP. However, TGF-β1 from megakaryocytes in CITP may not participate in the mechanism of thrombocytopenia and megakaryocyte maturation.