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目的：通过雷公藤孵化皮片，在同种小鼠间进行移植，探讨雷公藤对免疫排斥反应的抑制作用。方法：采用１５％雷公藤煎剂和４０μｇ／ｍｌ、１００μｇ／ｍｌ、５００μｇ／ｍｌ雷公藤总甙对供鼠皮片浸泡孵化，在昆明种小鼠和ＤＢＡ褐色小鼠间进行皮片移植，观察雷公藤对同种皮片移植免疫排斥的抑制作用。结果：雷公藤煎剂孵化皮片能显著延长移植物的存活时间，两种小鼠皮片移植后存活时间分别为（２２３１±３６０）天、（２１５０±３４０）天，与对照组（１３２０±２２５）天、（１１８１±２７５）天相比有显著意义（Ｐ＜００１）；４０μｇ／ｍｌ雷公藤总甙孵化后皮片存活时间为（１７５４±１４４）天，１００μｇ／ｍｌ为（１９８４±２６３）天，５００μｇ／ｍｌ为（２０９１±３８０）天；雷公藤总甙孵化加腹腔注射，每天４０μｇ／２０ｇ小鼠为（２１３５±２０８）天，１００μｇ为（２２３４±２７５）天，５００μｇ为（１９８２±１１４）天，与对照组（１２８３±０８０）天、（１１９６±１７５）天相比均有显著差异（Ｐ＜００１）；雷公藤总甙单纯孵化各组与孵化加腹腔注射相比，除４０μｇ／ｍｌ组（Ｐ＜００５）外，存活时?
OBJECTIVE: To study the inhibitory effect of tripterygium wilfordii on immune rejection by using tripterygium wilfordii to incubate skin slices and transplanting the same kind of mice. Methods: 15% tripterygium wilfordii decoction and 40μg / ml, 100μg / ml, 500μg / ml Tripterygium glycosides glycosides on mouse skin soak incubation, in Kunming mice and DBA brown mice skin graft was observed Inhibitory effects of Tripterygium wilfordii on immune rejection of the same skin graft. Results: Tripterygium wilfordii decoction incubated hatching skin could significantly prolong the survival time of the grafts. The survival time of the two kinds of skin grafts were (2231 ± 3.60) days, (2150 ± 3.40) Day, compared with the control group (1320 ± 2.25 days) and (1181 ± 275) days (P <001); 40μg / ml Tripterygium glycosides after hatching skin flap Survival time was (1754 ± 144) days, 100μg / ml was (1984 ± 263) days, 500μg / ml was (2091 ± 380) days; Tripterygium glycosides hatched plus Intraperitoneal injection of 40μg / 20g mouse daily (2135 ± 208) days, 100μg (2234 ± 275) days, 500μg (19 82 ± 1 14 days, compared with the control group (1283 ± 080) days, (1196 ± 175) days were significantly different (P <001); Tripterygium total glycosides incubated with each group and Of intraperitoneal injection when compared, in addition to 40μg / ml group (P <005), survival?