目的探索解偶联蛋白1(UCP1)基因启动子甲基化水平与肥胖的关系。方法选取某医院腹部手术患者116例,超重/肥胖组50例(BMI≥24.0),正常体重组66例(18.5≤BMI<24),提取两组白细胞DNA并进行甲基化处理,采用质谱法定量检测UCP1基因甲基化水平,比较两组甲基化差异及各CpG位点的甲基化水平与BMI的关系。结果 t检验结果显示,正常体重组和超重/肥胖组的各CpG位点的差异无统计学意义(P>0.05);多重线性回归分析发现,位点UCP1-2_CpG_10.11.12.13与BMI存在线性关系(回归系数为15.370,P<0.05)。结论 UCP1基因甲基化可能与肥胖存在一定关系。 Objective To explore the relationship between promoter methylation of UCP1 gene and obesity. Methods A total of 116 patients underwent abdominal surgery in a hospital, including 50 patients with overweight / obesity (BMI≥24.0) and 66 patients with normal weight (18.5≤BMI <24). Two groups of leukocyte DNA were extracted and methylated. Mass spectrometry The methylation level of UCP1 gene was quantitatively detected, and the methylation difference and the methylation level of each CpG site were compared with BMI. Results The results of t-test showed that there was no significant difference in CpG loci between normal weight group and overweight / obesity group (P> 0.05). Multiple linear regression analysis showed that there was a linear relationship between UCP1-2_CpG_10.11.12.13 and BMI Relationship (regression coefficient was 15.370, P <0.05). Conclusion UCP1 gene methylation may be related to the existence of obesity.