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以新疆野生樱桃李(Prunus sogdiana Vassilcz.)扦插苗为试材,通过人工接种南方根结线虫(Meloidogyneincognita)鉴定其抗性,同时对抗病与感病单株在接种后的酶活性变化以及根结的形成发育过程进行了研究。结果表明:新疆野生樱桃李对根结线虫抗性发生分离,大部分单株表现为感病,只有28%单株表现为抗病,并且其抗性与本试验室之前克隆得到的抗根结线虫相关基因psoRPM1的表达存在着相关关系。接种根结线虫后CAT、POD酶在抗病植株中的活性高于感病植株,说明它们可能参与了抗根结线虫的生理过程,而PPO和PAL活性则在接种后7～14日抗病植株高于感病植株,之后在两者之间差异不大。接种根结线虫后线虫由皮层进入中柱,并在10d时观察到形成了大量多核的巨型细胞,随后巨型细胞经过发育到接种后22d可观察到根系开始出现明显的根结。接种后30d观察到巨型细胞出现空洞。
The seedlings of Prunus sogdiana Vassilcz. Were used as tested materials, and their resistances were identified by artificial inoculation with Meloidogyne incognita. Meanwhile, the changes of enzyme activities of resistant and susceptible plants after inoculation, The formation and development of knot were studied. The results showed that Xinjiang wild cherry plum had the resistance to Meloidogyne incognita, most of the plants showed susceptible disease, and only 28% of the plants showed disease resistance, and its resistance was similar to that of the root knot The expression of nematode related gene psoRPM1 is correlated. After inoculated with root-knot nematode CAT, POD enzyme activity in resistant plants was higher than susceptible plants, indicating that they may be involved in the physiological processes of root-knot nematode, and PPO and PAL activity in 7 to 7 days after inoculation disease resistance Plants were higher than susceptible plants, with little difference between the two. After inoculation of root-knot nematode, the nematode entered the middle column from the cortex, and at 10 days it was observed that a large number of multinucleated giant cells were formed. After that, giant root cells were observed to develop obvious roots at 22 days after inoculation. After 30 days of inoculation, giant cells were observed to be hollow.